Brain hormones and appetite in fish

鱼的大脑激素和食欲

基本信息

批准号：
16380139
负责人：
TAKAHASHI Akiyoshi
金额：
$ 10.05万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

1. Melanin-concentrating hormone (MCH) of the barfin flounder is composed of 17 residues, which differs from salmon MCH at a position 2. The biological activity of synthetic MCH was demonstrated by the aggregation of melanosomes in flounder skin after intraperitoneal injection of the peptide. The number of MCH mRNA copies was greater in fish reared with a white background than in those reared with a black background. The MCH gene expression level in the brain was higher in fasted fish than in controls, indicating the association of MCH with food intake.2. We cloned two MCH receptors (MCH-R) from the barfin flounder. The expression of MCH-R1 was confined to the brain, while transcripts of MCH-R2 were detected in several tissues including the brain. The expression of MCH-R2 in eyed-sideskin was higher in fish reared with a black background than with a white background.3. In goldfish, cumulative food intake was decreased by intracerebroventricular injection of MCH, while it was increased by intracerebroventricular injection of MCH antiserum. The number of MCH immunoreactive somata in a part of the goldfish hypothalamus decreased significantly in fasted fish as compared with fed fish. These results indicate that MCH has a potential to function as an anorexigenic neuropeptide in goldfish.4. We cloned three POMC subtypes from the barfin flounder. The expression of POMC-A was confined to the pituitary, while transcripts of POMC-B and-C were detected in several tissues including the pituitary. Posttranslational processing of POMC of the pars distalis and pars intermedia in the barfin flounder pituitary differs from each other.5. Expressions of melanocortin 1 and 4 receptors were observed in the brain, eye-balls, testes, and skin of the barfin flounder. Melanocortin 4 receptor was also expressed in the liver, of which expression was increased by fasting. The results suggest the association of this receptor with energy homeostasis.

1。Barfin比目鱼的黑色素浓缩激素（MCH）由17个残基组成，该残基与鲑鱼MCH在2的位置不同。合成MCH的生物学活性通过腹膜内注射后比目鱼皮肤中黑色素体的聚集在肽腹膜内的皮肤中。用白色背景饲养的鱼类的MCH mRNA副本的数量要比在饲养黑色背景的鱼类背景中更大。在禁食鱼中，大脑中的MCH基因表达水平高于对照组中的MCH基因表达水平，表明MCH与食物摄入量的关联2。我们从Barfin Flounder克隆了两个MCH受体（MCH-R）。 MCH-R1的表达仅限于大脑，而MCH-R2的转录本在包括大脑在内的多个组织中被检测到。在饲养黑色背景的鱼中，眼睛侧皮中MCH-R2的表达高于白色背景3。在金鱼中，脑室内注射MCH会降低累积食物摄入，而脑室内注射MCH抗血清会增加。与喂养的鱼相比，在禁食鱼中，金鱼下丘脑的MCH免疫反应性somata数量显着减少。这些结果表明，MCH具有在金鱼中充当厌食的神经肽的潜力。4。我们从Barfin Flounder克隆了三个POMC亚型。 POMC-A的表达仅限于垂体，而在包括垂体在内的几个组织中检测到了POMC-B和C的转录本。 Barfin Flounder垂体中PARS远端的POMC和PARS INMEDIA的POMC的翻译后处理与彼此不同。5。在大脑，眼球，睾丸和皮肤的barfin比目鱼中观察到黑色皮质素1和4受体的表达。黑素皮质4受体也在肝脏中表达，禁食增加了表达。结果表明该受体与能量稳态的关系。

项目成果

期刊论文数量（78）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Immunocytochemical localization and ontogenic development of a-melanocyte-stimulating hormone (α-MSH) in the brain of a pleuronectiform fish, barfin flounder

DOI：
10.1007/s00441-004-1058-4
发表时间：
2005-04-01
期刊：
CELL AND TISSUE RESEARCH
影响因子：
3.6
作者：
Amano, M;Takahashi, A;Yamamori, K
通讯作者：
Yamamori, K

Central administration of melanin-concentrating hormone (MCH) suppresses food intake, but not locomotor activity, in the goldfish, Carassius auratus

DOI：
10.1016/j.neulet.2006.02.005
发表时间：
2006-05
期刊：
Neuroscience Letters
影响因子：
2.5
作者：
K. Matsuda;S. Shimakura;K. Maruyama;T. Miura;M. Uchiyama;H. Kawauchi;S. Shioda;A. Takahashi
通讯作者：
K. Matsuda;S. Shimakura;K. Maruyama;T. Miura;M. Uchiyama;H. Kawauchi;S. Shioda;A. Takahashi

Feeding-induced changes of melanin-concentrating hormone (MCH)-like immunoreactivity in the goldfish brain

进食引起的金鱼大脑中黑色素浓缩激素（MCH）样免疫反应性的变化