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自己会合性マメ科樹皮レクチンの液胞機能との関わり

自缔合豆科植物树皮凝集素与液泡功能的关系

基本信息

批准号：
02J01013
负责人：
上田晴子
金额：
$ 2.5万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02J01013/
关键词：
レクチン自己会合液胞エンジュシロイヌナズナ

项目摘要

マメ科植物は,さまざまな器官に多量の糖鎖認識タンパク質(レクチン)を蓄積している.研究者らは,マメ科樹木エンジュ(Sophora japonica)の樹皮レクチンsophoragrinが,自己の糖鎖を介して可溶性四量体と不溶性多量体を相互変換するという独自の性質を持つことを見い出した.この相互変換は,糖およびカルシウム濃度によって調節されていた.エンジュ樹皮組織の免疫染色から,sophoragrinが師部柔組織の液胞に多量に蓄積されていることが示された.液胞タンパク質は多様な経路で液胞へ輸送されることが知られているため,sophoragrinの輸送経路とレクチン活性を獲得する段階がsophoragrinの機能解明への手がかりになると考えられる.本研究では,sophoragrinの液胞輸送ならびに生合成経路を解析した.1.Sophoragrinの液胞輸送経路の解析Sophoragrinと緑色蛍光タンパク質(GFP)との融合タンパク質を恒常的に発現するタバコ培養細胞(BY-2)を作製し,融合タンパク質の局在を蛍光顕微鏡で観察した.その結果,小胞体および液胞以外に顆粒状の蛍光シグナルが得られた.種々のオルガネラマーカーと比較しながら,この顆粒の同定を進めている.2.Sophoragrin生合成の解析エンジュ樹皮から抽出液を調製し,細胞分画を行った.低分子量の成熟型sophoragrinは可溶性画分で検出されることから,液胞で成熟型への切断が起こることが示唆された.一方,高分子量前駆体sophoragrinは可溶性画分と不溶性画分で検出されたが,可溶性画分で検出される前駆体のみが糖結合性を示したことから,sophoragrinは液胞で糖結合性を獲得すると考えられた.

There is a large amount of sugar in the organs of the family Linnaeus. The researchers reported that the researchers, in the department of science and technology, were involved in the production of sugar, soluble tetra-body, insoluble multi-body, soluble quadruplet, insoluble multi-body, insoluble Let's talk to each other, sugar, sugar and sugar. The skin tissue was stained by immunostaining, and the liquid cells of sophoragrin tissue were stained by immunostaining, and the liquid cells were stored in large quantities. The liquid cell has a lot of information, and the liquid cell has a lot of information. The sophoragrin machine is able to understand the performance of the sophoragrin machine. In this study, the sophoragrin liquid cell was used to synthesize the liquid cell. 1.Sophoragrin liquid cell was used to analyze the Sophoragrin color, the light intensity (GFP), the fusion cell (GFP), the constant cell growth rate (BY-2), and the fusion signal. The results showed that the small cell body was fine outside the liquid cell, and the light was fine. A variety of chemicals were used to compare the results, and the results were compared. 2. Sophoragrin was synthesized and analyzed. The extraction solution was analyzed, and the cells were drawn separately. The soluble picture of low molecular weight "mature sophoragrin" showed that the liquid cell was mature, and the liquid cell was cut off. On one side, the high molecular weight precursor sophoragrin soluble picture showed that the soluble picture was insoluble, the soluble picture showed that the sugar binding property of the high molecular weight precursor, the sugar binding property of the sophoragrin precursor, and the sugar binding property of the high molecular weight precursor, showed that the sugar binding property of the high molecular weight precursor was not soluble, and the sugar binding property of the high molecular weight precursor was determined.