Analysis of gene expressions involved in cancer-host cells cross talk at the invasion front of nonsmall cell lung cancer
非小细胞肺癌侵袭前沿癌症与宿主细胞串扰相关基因表达分析
基本信息
- 批准号:13670192
- 负责人:
- 金额:$ 2.11万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
It is recently proposed that cancer cells make microenvironment suitable for their invasion through cross talk with host cells. To elucidate genes or molecules involved in the cancer cell-host cell cross talk at the invasion front of nonsmall cell lung cancer (NSCLC), mRNA profilings of 1176 cancer-related genes at the invasion front tissues of NSCLCs of various progressions were analyzed by cDNA macroarray system (Cancer array 1.2, Clontech). 121 genes, including cyclin D1, c-myc, insulin-like growth factor, rho-associated GTP binding proteins, laminin receptor, DNA polymerases, beta-catenin, wnt 8B, elongation factor alpha-1, were commonly overexpressed in NSCLC tissues. Ten genes, such as membrane-type 1 matrix metalloproteinase (MT1-MMP), MMP-3, p21-rac1, vascular endothelial growth factor (VEGF), jagged-1, jagged-2, notch-4, c-fos related antigen, were overexpressed both in squamous cell carcinomas and high stage-adenocarcinomas compared with low stage-adenocarcinomas, while ezrin (villin 2) and macrophage inhibitory cytokine (MTC)-1 genes did the opposite. These results were confirmed by real time RT-PCR using TaqMann-probe method. These data suggested that rac-1/MT1-MMP, jagged-1, -2/ notch-4, p53 / MIC-1 groups may actively involved in the cross talk between lung cancer and host cells and works either positively or negatively to make appropriate microenvironment for invasion of the lung cancer cells. We have also established laser-captured microdissection / real time RT-PCR method in order to analyze gene expressions more in detail at the invasion front of NSCLCs. Furthermore, functional and non-invasive examination methods of gene expressions in NSCLC tissues in vivo were tried successfully using FDG- and MIBI-PET. Present results may contribute to estimate biological features of NSCLCs and establish adequate therapy for lung cancer patients.
最近提出,癌细胞通过与宿主细胞的串扰来制造适合其侵袭的微环境。为了阐明参与非小细胞肺癌(NSCLC)侵袭前沿癌细胞-宿主细胞串扰的基因或分子,通过cDNA宏阵列系统(Cancer array 1.2,Clontech)分析了不同进展的NSCLC侵袭前沿组织中1176个癌症相关基因的mRNA谱。121个基因,包括cyclin D1、c-myc、胰岛素样生长因子、rho-associated GTP binding proteins、层粘连蛋白受体、DNA聚合酶、β-catenin、wnt 8B、延伸因子α-1,在NSCLC组织中普遍过表达。膜型基质金属蛋白酶1(MT 1-MMP)、MMP-3、p21-rac 1、血管内皮生长因子(VEGF)、jagged-1、jagged-2、notch-4、c-fos相关抗原等10个基因在鳞癌和高分期腺癌中的表达均高于低分期腺癌,而ezrin(villin 2)和巨噬细胞抑制因子(MTC)-1基因的表达则相反。这些结果通过使用TaqMann探针法的真实的时间RT-PCR证实。这些数据表明,rac-1/MT 1-MMP,jagged-1,-2/ notch-4,p53 / MIC-1基团可能积极参与肺癌与宿主细胞之间的串扰,并积极或消极地为肺癌细胞的侵袭创造适当的微环境。我们还建立了激光捕获显微切割/真实的时间RT-PCR方法,以便更详细地分析NSCLC侵袭前沿的基因表达。此外,功能和非侵入性的检测方法在体内的基因表达在NSCLC组织中成功地尝试使用FDG-和MIBI-PET。本研究结果可能有助于评估非小细胞肺癌的生物学特性,并建立适当的治疗肺癌患者。
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kotaro Higashi et al.: "Value of whole-body FDG PET in management of lung cancer"Ann. Nucl. Med.. 17・1. 1-14 (2003)
Kotaro Higashi 等:“全身 FDG PET 在肺癌治疗中的价值”Ann. 17・1 (2003)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Jinshan Zhou et al.: "Expression of multidrug resistence protein and mRNA correlate with 99mTc-MIBI imaging in patients with lung cancer"J. Nucl. Med.. 42・10. 1476-1483 (2001)
Jinshan Zhou 等:“肺癌患者中多药耐药蛋白和 mRNA 的表达与 99mTc-MIBI 成像相关”J. Nucl. 1476-1483 (2001)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Jinshan Zhou: "Expression of multidrug resistence protein and mRNA correlate with 99mTc-MIBI imaging in patients with lung cancer"J. Nuci. Med.. 42・10. 1476-1483 (2001)
周金山:“肺癌患者中多药耐药蛋白的表达及其与 99mTc-MIBI 成像的相关性”J. Nuci. 1476-1483 (2001)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Jinshan Zhou, Kotaro Higashi, Yoshimichi Ueda, Yuko Kodama, Dachuan Guo, Fumiko Jisaki, Aya Sakurai, Tsutomu Takegami, Shogo Katsuda, and Itaru Yamamoto: "Expression of multidrug resistance protein and messenger RNA correlate with 99mTc-MIBI imaging in pa
Jinshan Zhou、Kotaro Higashi、Yoshimichi Ueda、Yuko Kodama、Dachuanuo、Fumiko Jisaki、Aya Sakurai、Tsutomu Takegami、Shogo Katsuda 和 Itaru Yamamoto:“多药耐药蛋白和信使 RNA 的表达与 99mTc-MIBI 成像相关
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kotaro Higashi, Ichiro Matsunari, Yoshimichi Ueda, Ryosuke Ikeda, Jinfei Guo, Manabu Oguchi, Hisao Tonami and Itaru Yamamoto: "Value of whole-body FDG PET in management of lung cancer."Ann. Nucl. Med.. 17(1). 1-14 (2003)
Kotaro Higashi、Ichiro Matsunari、Yoshimichi Ueda、Ryosuke Ikeda、Jinfeiuo、Manabu Oguchi、Hisao Tonami 和 Itaru Yamamoto:“全身 FDG PET 在肺癌治疗中的价值。”Ann。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
UEDA Yoshimichi其他文献
UEDA Yoshimichi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('UEDA Yoshimichi', 18)}}的其他基金
Is sphingolipid of the cell membrane involved in invasion and metastasis of non-adenocarcinoma of the lung?
细胞膜鞘脂是否参与肺非腺癌的侵袭和转移?
- 批准号:
18K07002 - 财政年份:2018
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
An approach to free independence based on mutual information
基于互信息的自由独立方法
- 批准号:
16K13762 - 财政年份:2016
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Is sphingolipid of the cell membrane involved in invasion and metastasis of adenocarcinoma of the lung?
细胞膜鞘脂是否参与肺腺癌的侵袭和转移?
- 批准号:
26460442 - 财政年份:2014
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on von Neumann algebras, free probability and non-commutative function spaces
冯诺依曼代数、自由概率和非交换函数空间的研究
- 批准号:
24540214 - 财政年份:2012
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Involvement and their roles of aquaporins in the metastasis and drug-resistance of bone and soft tissue sarcomas
水通道蛋白在骨软组织肉瘤转移和耐药中的参与及其作用
- 批准号:
22590323 - 财政年份:2010
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on free probability and operator algebras
自由概率与算子代数研究
- 批准号:
20540213 - 财政年份:2008
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of activation mechanism of HMGA2 gene and its regulating gene-pathways in the progression of lung cancer
HMGA2基因在肺癌进展中的激活机制及其调控基因通路分析
- 批准号:
19590370 - 财政年份:2007
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of activated gene network in the microenvironment of lung cancer-invasion front.
肺癌侵袭前沿微环境激活基因网络分析
- 批准号:
17590320 - 财政年份:2005
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Expression profiling of genes involved in tumor - host stroma cell interaction in the metastasis of human fibrosarcoma cell (HT1080)
人纤维肉瘤细胞(HT1080)转移中涉及肿瘤-宿主基质细胞相互作用的基因的表达谱
- 批准号:
15590322 - 财政年份:2003
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanism of immune escape of human osteosarcoma cells through abnormal expressions of Fas and Fas ligand.
人骨肉瘤细胞通过Fas和Fas配体表达异常的免疫逃逸机制。
- 批准号:
11670198 - 财政年份:1999
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
The role and interrelationship of PRDM1 and p53 in the regulation of differentiation, invasion, and metastasis of hepatocellular carcinoma
PRDM1和p53在肝细胞癌分化、侵袭和转移调控中的作用及相互关系
- 批准号:
23K15048 - 财政年份:2023
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
The role of regulation and subcellular localization of GTP biosynthesis in melanoma invasion and metastasis
GTP生物合成的调控和亚细胞定位在黑色素瘤侵袭和转移中的作用
- 批准号:
10636058 - 财政年份:2023
- 资助金额:
$ 2.11万 - 项目类别:
Deciphering epigenetically-regulated pathways to improve targeted therapy for invasion and metastasis in head and neck cancer
破译表观遗传调控途径以改善头颈癌侵袭和转移的靶向治疗
- 批准号:
10650527 - 财政年份:2023
- 资助金额:
$ 2.11万 - 项目类别:
The role of MIRO2 in tumor cell invasion and metastasis
MIRO2在肿瘤细胞侵袭和转移中的作用
- 批准号:
10704512 - 财政年份:2022
- 资助金额:
$ 2.11万 - 项目类别:
The role of FMNL2 in invasion and metastasis
FMNL2在侵袭和转移中的作用
- 批准号:
462700 - 财政年份:2022
- 资助金额:
$ 2.11万 - 项目类别:
Operating Grants
The role of MIRO2 in tumor cell invasion and metastasis
MIRO2在肿瘤细胞侵袭和转移中的作用
- 批准号:
10464387 - 财政年份:2022
- 资助金额:
$ 2.11万 - 项目类别:
Development of a glioblastoma treatment based on CLIC2, a protein that inhibits invasion and metastasis of malignant tumors
基于CLIC2(一种抑制恶性肿瘤侵袭和转移的蛋白质)开发胶质母细胞瘤治疗方法
- 批准号:
21K16611 - 财政年份:2021
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Crosstalk between Netrin-1 and BMP regulates the invasion and metastasis of melanoma
Netrin-1与BMP的串扰调控黑色素瘤的侵袭和转移
- 批准号:
21K10260 - 财政年份:2021
- 资助金额:
$ 2.11万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigating the Regulation of RET Receptor-Mediated Perineural Invasion and Metastasis
RET 受体介导的神经周围侵袭和转移的调控研究
- 批准号:
444955 - 财政年份:2021
- 资助金额:
$ 2.11万 - 项目类别:
Operating Grants
Targeting radiation driven invasion and metastasis in cancers of unmet need
针对未满足需求的癌症中的辐射驱动的侵袭和转移
- 批准号:
MR/T04358X/1 - 财政年份:2021
- 资助金额:
$ 2.11万 - 项目类别:
Fellowship