REGULATION OF TGF_ACTIVITY FOR ATTENUATION OF GLOMERULAR LESIONS

调节 TGF_活性以减弱肾小球病变

• 批准号: 13671133
• 负责人: OKUDA Seiya
• 金额: $ 2.18万
• 依托单位: KURUME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671133/
• 关键词: renal regeneration; angiogenesis; vasculogenesis; glomerulosclerosis; CD34 positive cell; tubulointerstitial fibrosis; endothelial cell; 腎組織; 再構築; 硬化; 線維化; 内皮前駆細胞; 分化誘導

Tissue injury of glomerulus and tubulointerstitium is followed by tissue regeneration in most of renal diseases. Recently, angiogenesis has been reported to play an important role in renal tissue regeneration. In experimental mesangial proliferative glomerulitis model (Thy-1 nephritis) angiogenesis is elevated in the repair course of tissue generation, while an insufficient angiogenesis resulted in irreversible tissue damage such as glomerulosclerosis and tubulointerstitial fibrosis. Angiogenesis have been thought tobe composed of in situ proliferation and migration of capillary endothelial cells in tissue regeneration. Recently, however, endothelial progenitor cells whichexist in adult have been reported to differentiate to capillary endothelial cells. Endothelial progenitor cells are CD34 positive. After culture of separated CD 34 positive cells, they showed appearance of endothelial cells which indicates that CD34 positive cells is related to angiogenesis. Thereafter angiogenesis in … More adult tissue regeneration is suggested not only in situ endothelial proliferation and migration as believed previously but also (differentiation of endothelial progenitor cell to endothelial cell) as seen in developmental period. The endothelial progenitor cells exists in peripheral blood, bone marrow and umbilical cord blood. We investigated whether it is possible that endothelial umbilical cord blood cells differentiate to endothelial progenitor cells and wether vasculogenesis by endothelial progenitor cells is involved in renal tissue regeneration in experimental glomerulonephritis. CD34 positive umbilical cord cells were injected into renal artery in thy-1 nephritis -induced rats. Injected CD34 positive cells existed in the injured glomerulus. However, the degree of glomerular injury was not different from the control nephritis at week 1 or two after injection. The study did not show the involvement of umbilical endothelial progenitor cells in renal tissue regeneration in experimental mesangial glomerulonephritis. Less

在大多数肾脏疾病中，肾小球和肾小管的组织损伤之后是组织再生。近年来，血管生成在肾组织再生中起着重要作用。在实验性系膜增生性肾小球炎模型（Thy-1肾炎）中，血管生成在组织生成的修复过程中升高，而血管生成不足导致不可逆的组织损伤，如肾小球硬化和肾小管间质纤维化。组织再生过程中，血管生成是由毛细血管内皮细胞的原位增殖和迁移组成的。然而，最近有报道称，存在于成人的内皮祖细胞可分化为毛细血管内皮细胞。内皮祖细胞是CD 34阳性的。分离的CD 34阳性细胞培养后出现内皮细胞，表明CD 34阳性细胞与血管生成有关。此后， ...更多信息 提示成人组织再生不仅如先前所认为的原位内皮增殖和迁移，而且如发育期所见的（内皮祖细胞向内皮细胞的分化）。内皮祖细胞存在于外周血、骨髓和脐带血中。我们研究了内皮脐带血细胞是否可能分化为内皮祖细胞，以及内皮祖细胞的血管生成是否参与了实验性肾小球肾炎的肾组织再生。将CD 34阳性脐带细胞注入Thy-1肾炎模型大鼠肾动脉。损伤的肾小球内存在CD 34阳性细胞。但在注射后1周或2周，肾小球损伤程度与对照组无差异。该研究并未表明脐内皮祖细胞参与实验性系膜肾小球肾炎的肾组织再生。少

项目成果

Okuda, S 他4名5番目: "Depressor effect by exercise training is associated with amelioration of hyperinsulinemia and sympathetic overactivity"Internal Medicine. 39. 1013-1019 (2000)

Okuda，S 和其他 4 人第 5 篇：“运动训练的降压作用与高胰岛素血症和交感神经过度活跃的改善有关”《内科医学》39. 1013-1019 (2000)。

Okuda, S 他7名8番目: "Ectopic expression of Smad7 inhibits transformationn growth favtor-b responses in vascular smooth muscle cells"Life Sciences. 69. 2641-2652 (2001)

Okuda，S 和其他 7 人第 8 篇：“Smad7 的异位表达抑制血管平滑肌细胞中的转化生长因子-b 反应”生命科学 69. 2641-2652 (2001)。

Okuda, S 他3名4番目: "Different effects of 22-oxacalcitrol and calcitriol on the course of experimental chronic renal failure"J Lab Clin Med. 140. 242-249 (2002)

Okuda，S 等 3 人第 4 篇：“22-奥沙骨化醇和骨化三醇对实验性慢性肾衰竭过程的不同影响”J Lab Clin Med. 140. 242-249 (2002)

Okuda, S 他7名8番目: "Ectopic expression of Smad7 inhibits transformation growth favtor-b responses in vascular smooth muscle cells"Life Sciences. 69. 2641-2652 (2001)

Okuda，S 和其他 7 人第 8 篇：“Smad7 的异位表达抑制血管平滑肌细胞中的转化生长因子-b 反应”生命科学 69. 2641-2652 (2001)。

Okuda, S 他12名13番目: "Caveolae integration of growth factor receptor in mesangial cells ans caveolin expression in mesangial proliferative glomerulonephritis"Kidney Int. 60,1. 471-480 (2001)

Okuda，S 和其他 12 位，第 13 期：“系膜细胞中的小窝整合和小窝蛋白在系膜增殖性肾小球肾炎中的表达”Kidney Int 60，1。