Analysis of the effects of motor command for vocalization upon auditory mechanisms in the brainstem

发声运动指令对脑干听觉机制的影响分析

• 批准号: 13671762
• 负责人: SAKAMOTO Takashi
• 金额: $ 2.3万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671762/
• 关键词: Vocalization; Periaqueductal gray; Auditory relay neuron

To investigate the effects of motor commands for vocalization upon auditory mechanisms in the brainstem during actual vocalization movement, we first identified the auditory relay neurons within the brainstem, and analyzed the inputs from the vocalization center and their discharge properties.In precollicular-postmammilary decerebrate cats, we employed the midbrain periaqueductal gray as the vocalization center, and analyzed the discharge properties of auditory relay neurons within the dorsal and ventral nuclei of the lateral remuniscus responding to the auditory stimuli that has the same fundamental frequency and formant as those of induced vocalization. These stimuli were reconstructed by the computer controlled apparatus that was equipped by this grant.We first tried to identify the responsibility to the single electrical shock delivered to the vocalization center and the responsibility to the different auditory stimuli, and then the discharge properties of these neurons during the actual vocalization movements.Most of recorded auditory relay neurons did not respond to the single shock delivered to the vocalization center within the stimulus strength which could induce vocalization movements. With higher stimulus strength, some neurons received suppressive effects and some were excitatory. However, these differences were not significant statistically, so that we did not published our results yet. To clarify the effects from the vocalization center, we are now trying to analyze the effects upon the discharges induced by the auditory stimuli. The analysis of discharge properties of auditory relay neurons during actual vocalization was not performed yet, because accompanying movements of brainstem was unexpectedly large so that stable recording was quite difficult.

为了探讨实际发声运动中发声运动对脑干听觉机制的影响，我们首先确定了脑干内的听觉中继神经元，并分析了发声中枢的输入及其放电特性。在丘前-乳后去脑猫中，我们以中脑导水管周围灰质为发声中枢，分析了与诱导发声具有相同基频和共振峰的听觉刺激时，外侧索状核背侧和腹侧核内听觉中继神经元的放电特性。我们首先尝试识别对单个电击传递给发声中枢的响应和对不同听觉刺激的响应，然后识别这些神经元在实际发声运动中的放电特性，记录到的大部分听觉中继神经元在刺激强度范围内对传递到发声中心的单次电击没有反应。随着刺激强度的增加，部分神经元受到抑制作用，一些神经元产生兴奋作用。然而，这些差异在统计学上并不显著，因此我们还没有公布我们的结果。为了阐明发声中枢的影响，我们现在正试图分析听觉刺激对放电的影响。在实际发声过程中，听觉中继神经元的放电特性还没有进行分析，因为脑干的伴随运动出人意料地大，因此稳定的记录相当困难。