Development of molecular probes for nucleic acids imaging based on 19F NMR/MRI
Development of molecular probes for nucleic acids imaging based on 19F NMR/MRI
批准号:
23750185
负责人:
SAKAMOTO Takashi
金额:
$2.91万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
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英文摘要
To improve the detection limit of the probe for 19F MR detection or imaging of target nucleic acids, new 19F labeling reagents were synthesized and introduced to the 5’ end of oligonucleotide probe having stem-loop structure. Results of the 19F NMR measurement in the presence of target DNA suggesting that the 19F labels having mono chiral structure have 5-fold large S/N ratio compared with racemic one, and the multi labeling is effective for improvement of detection limit. Furthermore, the probe capable of ratiometric detection of target DNA was successfully developed.
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5-Methylcytosine selective photoligation using photoresponsive oligonucleotides containing various 5-vinyl-2-deoxyuridines having an aromatic group
使用含有各种具有芳香基团的 5-乙烯基-2-脱氧尿苷的光响应寡核苷酸进行 5-甲基胞嘧啶选择性光连接
DOI:
--
发表时间:
2012
期刊:
Chemistry Letters
影响因子:
1.6
作者:
[Takashi Sakamoto, Takehiro Ami, Kenzo Fujimoto]
通讯作者:
Kenzo Fujimoto
核酸の分子認識を利用した新規フッ素核磁気共鳴イメージングプローブの創製
利用核酸分子识别创建新型氟核磁共振成像探针
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[坂本隆, 早川輝, 清水勇喜, 佐々木淳, 藤本健造]
通讯作者:
藤本健造
フッ素核磁気共鳴によるカリウムイオンセンサーの開発
氟核磁共振钾离子传感器的研制
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[早川輝, 佐々木淳, 坂本隆, 藤本健造]
通讯作者:
藤本健造
Quick Regulation of mRNA Functions by a Few Seconds of Photoirradiation,
通过几秒钟的光照射快速调节 mRNA 功能,
DOI:
10.1039/c2ob25883h
发表时间:
2012
期刊:
Organic & Biomolecular Chemistry
影响因子:
3.2
作者:
[Kenzo Fujimoto, Hiroki Yoshinaga, Yasumasa Yoshio and Takashi Sakamoto, Atsuo Shigeno]
通讯作者:
Atsuo Shigeno
カリウムイオンをセンシングする19F MRI 用造影プローブの開発
开发感应钾离子的 19F MRI 对比探针
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[早川輝, 佐々木淳, 坂本隆, 藤本健造]
通讯作者:
藤本健造
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国内基金
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