Neuroprotective Effects of some drugs on adult rat brain following central auditory tract axotomy

一些药物对中央听觉道轴索切除术后成年大鼠大脑的神经保护作用

• 批准号: 13671774
• 负责人: ITO Makoto
• 金额: $ 2.5万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671774/
• 关键词: Commissure of Probst; Facial nerve axotomy; erythropoietin; Toki-shakuyaku-San; G-CSF; Free radical scavenger; ビタミンE; エリスロポエイン; TJ-23; 当帰芍薬散(TJ-23); プロブスト交連; 外側毛帯背側核; 中枢性聴覚障害; 神経再生・変性

oxidative stress and massive production of oxygen-derived free radicals such as nitric oxide(NO) might be one of the most important mechanisms of neuronal cell death following central and/or peripheral nerve injury. This study was aimed to investigate whether recombinant human erythropoietin(rhEPO) and granulocyte colony stimulating factor(G-CSF) exert their neuroprotective effect on the lesioned facial nucleus(FMN) and the dorsal nucleus of lateral lemniscus(DNLL) after nerve injury in the brainstem.1.After the facial nerve axotomy in the brainstem according to stereotaxic coordinates, the survival ratio of motoneurons in the FMN was 25.0±2.4% on day 14 and 2.8±1.4% on day 28 compared to the contralateral intact side. The survival ratio of motoneurons in the FMN with the rhEPO administration (5000 U/kg daily) was 42.6±6.7% on day 14 and 8.2±0.5% on day 28.2.The commissure of Probst was transected through its midline decussation with micro knife inserted into the brain according to stereotaxic coordinates. Most of the contralaterally projecting neurons of the DNLL underwent retrograde degeneration on day 14. The survival ratio of the DLL nuerons with the G-CSF administration (50ug/kg daily) was 50% on day 14.These findings suggest that rhEPO and G-CSF have neuroprotective property to the retrograde degeneration of the FMN and the DNLL after axotomy in the brainstem.

氧化应激和氧源性自由基如一氧化氮（NO）的大量产生可能是中枢和/或外周神经损伤后神经元细胞死亡的最重要机制之一。本研究旨在探讨重组人促红细胞生成素（rhEPO）和粒细胞集落刺激因子（G-CSF）对脑干神经损伤后受损面神经核（FMN）和外侧丘系背核（DNLL）的神经保护作用。与健侧相比，FMN中运动神经元的存活率在第14天和第28天分别为25.0±2.4%和2.8±1.4%。注射rhEPO（5000 U/kg/d）后，FMN内运动神经元存活率在第14天为42.6±6.7%，第28天为8.2±0.5%。大部分的对侧投射神经元的DNLL进行逆行变性的第14天。给予G-CSF（50 ug/kg/d），第14天DLL神经元的存活率为50%。这些结果表明rhEPO和G-CSF对脑干轴突切断后FMN和DNLL的逆行变性具有神经保护作用。

项目成果

ラット脳幹内軸索損傷により惹起される顔面神経の逆行性変性に対するエリスロポエチンの神経保護効果の検討

促红细胞生成素对大鼠脑干轴突损伤所致面神经退行性变的神经保护作用研究

• 发表时间: 2004
• 期刊: 金沢十全医学会雑誌 113(1)
• 作者: 東 良

Successful Treatment of Hemifacial Spasm With Selective Facial Nerve Block Usine Doxorubicin(Adriamycin) under Local Anesthesia

局部麻醉下使用阿霉素选择性面神经阻滞成功治疗面肌痉挛

• 发表时间: 2004
• 期刊: Acta Otolaryngol 124
• 作者: 東 良;Makoto Ito
• 通讯作者: Makoto Ito

Degeneration of facial nucleus following the nerve transection in brainstem

脑干神经横断后面神经核变性

• 发表时间: 2001
• 期刊: Neurotrauma Res Vol.13
• 作者: 東 良;Makoto Ito;Tsuyoshi Mashitani
• 通讯作者: Tsuyoshi Mashitani