权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of artificial cornea utilizing with Apatite-polymer composites technique and nano-micro-fabrication technique

利用磷灰石-聚合物复合技术和纳米微加工技术开发人工角膜

基本信息

批准号：
15300176
负责人：
KOBAYASHI Hisatoshi
金额：
$ 10.5万
依托单位：
National Institutes for Materials Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In this study, we synthesized the composites of the macromolecule - protein which controlled nano-structure to use for an optics part and composites such as macromolecule - collagen - hydoxyapatite complexes to use for a peripheral part of the corneal prosthesis, and accumulated the fundamental data as materials for artificial cornea. We developed surface modification technique of the PVA hydro-gel by the bio-molecules such as collagen, RGDS, IKVAV to improve the biocompatibility. And we also developed the new high molecular weight Poly(malic acid) derivatives as a surface modifier and polysaccharide and amino acids immobilization technique as a artificial ligand to enhance the biological responses.To achieve the strong fixation of the artificial corneal devices on the host cornea, we developed the biocompatible PVA-hydroxyapatite composites. We have developed two different methods, alternate soaking method and particles kneading method, to make the PVA-hydroxyapatite composites. We ha … More ve succeeded to introduce the porous structure into the PVA-hydroxyapatite composites by using micro-fabrication technique. We utilized the perforation technology and nanofiber nonwoven fabric prepared by the electrospinning methods to give the 3D structure and inspected whether an effect of physical anchoring contributed to stability of epidermis.We started the materials evaluation by using primary cells from rabbit cornea and the feasibility study on the rabbits cornea during the periods. Stratifications of epidermis were confirmed on the PVA-collagen composites as a material for optical part. In addition, the cell adhesion and spreading that agreed with density of a ligand molecule were confirmed.The adhered cells were stable for a long term on the adhesion protein immobilized nanofiber sheet. And clear affinity was given for the cornea cells on the collagen immobilization PVA hydro-gel-hydroxyapatite composites, The surface topology clearly influenced the epithelialization on an artificial cornea and the stabilization greatly, and a big indicator for a design of the artificial cornea which functioned for a long term was provided. As a result of implantation of PVA-HAp composites in the rabbit cornea, an invasion of a blood vessel and host tissues into the micropore structure were observed and the results suggested that the strong fixation of a device was realized was expected. Less

在本研究中，我们合成了用于光学部分的高分子-蛋白质控制纳米结构的复合材料和用于角膜假体外围部分的高分子-胶原-羟基磷灰石复合物等复合材料，积累了作为人工角膜材料的基础数据。采用胶原蛋白、RGDS、IKVAV等生物分子对PVA水凝胶进行表面改性，提高其生物相容性。同时，我们还开发了新的高分子量聚苹果酸衍生物作为表面改性剂，以及多糖和氨基酸作为人工配体的固定技术来增强生物反应。为了实现人工角膜装置在宿主角膜上的强固定，我们研制了生物相容性pva -羟基磷灰石复合材料。采用交替浸渍法和颗粒揉捏法制备pva -羟基磷灰石复合材料。通过微加工技术，我们成功地将多孔结构引入pva -羟基磷灰石复合材料中。我们利用穿孔技术和静电纺丝法制备的纳米纤维非织造布，给出了三维结构，并考察了物理锚定对表皮稳定性的影响。我们从兔角膜原代细胞开始材料评价，并在此期间对兔角膜进行可行性研究。作为光学部件的材料，pva -胶原复合材料表面存在表皮层状结构。此外，还证实了细胞的粘附和扩散与配体分子的密度一致。在黏附蛋白固定的纳米纤维片上，黏附细胞长期稳定。胶原固定化聚乙烯醇-凝胶-羟基磷灰石复合材料对角膜细胞有明显的亲和性，其表面拓扑结构对人工角膜的上皮化和稳定性有明显的影响，为设计长期使用的人工角膜提供了重要的指标。PVA-HAp复合材料植入兔角膜后，观察到血管和宿主组织侵入微孔结构，结果表明该装置实现了预期的强固定。少

项目成果

期刊论文数量（159）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Study on the hydrolytic degradation of poly(α, β-malic acid) by direct polycondensation

DOI：
10.1016/j.msec.2004.08.029
发表时间：
2004-12
期刊：
Materials Science and Engineering: C
影响因子：
0
作者：
T. Kajiyama;Hisatoshi Kobayashi;T. Taguchi;Y. Komatsu;K. Kataoka;J. Tanaka
通讯作者：
T. Kajiyama;Hisatoshi Kobayashi;T. Taguchi;Y. Komatsu;K. Kataoka;J. Tanaka

Mulitiarray Formation of Cell Spherolds on a Microfabricated PEG-Brush Surface and Their Stabilization of Tissue Like Functions.

微加工 PEG 刷表面细胞球体的多阵列形成及其组织样功能的稳定性。

DOI：
发表时间：
2006
期刊：
Bioindustry 23(1)
影响因子：
0
作者：
Otsuka;H.;Kataoka;K.
通讯作者：
K.

Transplantation of corneal endothelium with descemet's membrane using a hydroxyethyl methacrylate polymer as a carrier

以甲基丙烯酸羟乙酯聚合物为载体的角膜内皮移植和后弹力层移植

DOI：
发表时间：
2005
期刊：
British Journal of Ophthalmology 89(2)
影响因子：
0
作者：
Shigeto Shimmura;Hideyuki Miyashita;Kenji Konomi;Tetsushi Taguchi;Hisatoshi Kobayashi;Jun Shimazaki;Junzo Tanaka;Kazuo Tsubota
通讯作者：
Kazuo Tsubota

Rapid and highly sensitive detection of cadmium chloride induced cytotoxicity using the HSP70B' promoter in live cells

使用 HSP70B 启动子在活细胞中快速、高灵敏地检测氯化镉诱导的细胞毒性