Ecological genetic studies on in-situ conservation of plant genetic resources in disturbed habitats

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In order to obtain the fundamental knowledge on in-situ conservation of plant genetic resources, field scientific approach, molecular phylogenetic approach and population biological approach were applied for describing the genetic and biological diversity of plants grown under disturbance. In East Asiatic plant species, we confirmed the association of dwarf type weedy oats in Yu-mai (naked oats) field in Yunnan, China, habitat segregation of Ceratotropis species (large or small population construction habit), adaptation to annual from perennial in a particular habitat in Echinochloa species, on-situ conservation of vulnerable species in traditional home garden, semi-cultivation of wild water pepper by expedition-base-survey on habitats of the wild relatives of cultivated plants. We developed SSR and SNP markers which are useful for ecological genetic study on ruderals. In phylogenetic studies, we determined geographical distribution of chloroplast DNA SNPs in Lilium, relationship of intertaxa of Symplocos, Aster ageratoides complex, Vitaceae species, Raphanus, Triticum-Aegilops complex, Cicer and diversity of SSR and ITS in Glycine and Echinochloa. In population dynamic studies, we characterize genetic diversity of natural populations under disturbance in wild azuki, wild soybean, Imperata, and composite species. We evaluated character state on each trees and/or geographical distribution and concluded that evolution of the traits unique to disturbed environment is fast with interaction between cultivated plant and its wild counterparts. In in-situ conservation of wild genetic resources, it is very important to gather the information on the genetic diversity created in prehistoric time, recent adaptation to habitat and maintenance mechanism of population