The basement study for PET: utilization of the information, which is obtained from bioradiographic study

正电子发射断层扫描（PET）等无创分子成像技术在疾病诊断和治疗中具有潜在的作用，但其诊断受到多种因素和问题的影响。为了验证PET诊断，对术前接受2-[^<18> F]氟-2-脱氧-d -葡萄糖(FDG)-PET检查的顽固性癫痫患者术中获得的人类新皮质脑切片进行了一种新的放射自显影检查（生物放射摄影）。在对照和高K^+条件下，人类和大鼠脑切片中FDG的摄取持续增加至250分钟。在对照培养条件下，白质对FDG的吸收高于灰质。在高K^+培养条件下，人和大鼠脑灰质对FDG的摄取均显著增加，而脑白质对FDG的摄取则无显著增加。对照组脑切片FDG摄取率与PET成像呈负相关，分别为同侧与对侧颞叶皮质（IC/CC） （r=0.936, p=0.0007）和同侧颞叶皮质与小脑（IC/CB）比值（r=0.788, p=0.0330）。相反，在高K^+条件下，它与PET呈弱正相关。FDG脑切片的摄取率表现为高K+/对照比，与FDG- pet观察到的IC/CC比（r=0.844, p=0.0134）和LC/CB比（r=0.904, p=0.0028）非常吻合。我们的实验系统应该为诊断和医学治疗提供直接有用的信息，也可以用于将体外和体内的研究结果联系起来，以及评估动物研究与人类的相关性。

Noninvasive molecular-imaging techniques such a positron emission tomography (PET) have potential roles in disease diagnosis and therapy, but its diagnosis is influenced by several factors and problems. In order to validate PET diagnosis, a novel autoradiographic examination (bioradiography was carried out in human neocortical brain slices obtained at operation from patients with intractable epilepsy who had received 2-[^<18> F]fluoro-2-deoxy-D-glucose (FDG)-PET examination pre-operatively.FDG uptake in human and rat brain slices under control and high K^+ conditions continuously increased up to 250 min. The white matter showed higher FDG uptake than gray matter under control culture condition. When the slices cultured under high K^+, FDG uptake in both human and rat gray matter were significantly enhanced, whereas that in the white matter did not. The uptake rates of FDG in brain slices under the control condition showed an inverse correlation with those seen in PET, which evaluated as ipsilateral and contralateral temporal cortex (IC/CC) (r=0.936, p=0.0007) and ipsilateral temporal cortex and cerebellum (IC/CB) ratio (r=0.788, p=0.0330). On the contrary, it showed a weak positive correlation with PET under the high K^+ condition. The uptake rates of FDG in brain slices expressed as high K+/control ratio, closely matched that observed by FDG-PET, which evaluated as the IC/CC ratio (r=0.844, p=0.0134) and the LC/CB ratio (r=0.904, p=0.0028).Our experimental system should provide information directly useful for diagnosis and medical treatment, as well as being useful for relating in vitro to in vivo findings, and for evaluating the relevance of animal studies to humans.