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Development of Low-temperature Sterilization Technique Using Microwave Plasma and Its Medical Application

微波等离子体低温灭菌技术的发展及其医学应用

基本信息

批准号：
16340180
负责人：
NAGATSU Masaaki
金额：
$ 9.79万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16340180/
关键词：
Plasma sterilization Surface wave plasma volume wave plasma Oxygen radicals Microwave plasma Low temperature sterilization Ultra violet emission Medical application プラズマ減菌パルス変調

项目摘要

In this research, we aimed at developing a novel low-temperature sterilization technique using large-volume microwave plasma and finally applying the results to the medical field. In the fiscal year 2004, we have carried out the experiments to clarify the mechanism of plasma sterilization and to study the possibility of improving the characteristics of rapid sterilization at low temperature. In the fiscal years 2005 and 2006, we have performed the experimental verification of low-temperature internal sterilization for the wrapped medical instruments using the volume-wave plasma in order to confirm the sterilization of specific medical equipments such as catheter. In the following, we listed the main results of the present research.(1) Clarification of the sterilization mechanism of oxygen plasma: It was experimentally confirmed that the etching effect due to the oxygen radicals was the main sterilizing factor in the oxygen plasma. While no strong UV emission was observed in the oxygen … More plasma, it was shown that the UV emissions were main factor in the nitrogen plasma, since there were several strong UV emissions in the range of 290〜400 nm.(2) Reduction of thermal effect by means of on-off control of microwave plasma: It is required to avoid thermal effect in the case of plastic medical instruments, because of non-heat resist materials. By introducing the time-modulated operation of microwave plasma, we succeeded to reduce the temperature of the samples less than 70 ℃.(3) Experiment of volume-wave plasma production: To sterilize inside the wrapped articles, we have carried out the sterilization experiment using the volume-wave plasma. We confirmed that the plasma was produced inside the plastic bottle. By using the pulsed-microwave plasma, we confirmed the sterilization of biological indicators (Bacillus Atrophaous) wrapped by glassine paper within 20 min.(4) Effect of OH radicals: We have investigated the effect of water vapor addition on the sterilization performance using the microwave plasma with air-simulated nitrogen-oxygen gas mixture. From the results, it was deduced that the water vapor played an important role on the improvement of sterilization speed. The collapse of spore cells might be attributed to the OH radicals generated in the plasma.(5) Summary of results: We reported 16 journal papers, 2 books, 38 proceeding of international conferences, and 132 presentations in the domestic meetings. We also applied 7 domestic and foreign patents relating with the present plasma sterilization, and we have taken one patent on the plasma sterilization method. Less

在本研究中，我们旨在开发一种新的低温灭菌技术，利用大体积微波等离子体，并最终将结果应用于医学领域。在2004财政年度，我们进行了实验，以阐明等离子体灭菌的机理，并研究提高低温快速灭菌特性的可能性。在2005和2006会计年度，为了确认导管等特定医疗设备的灭菌，我们对包装医疗器械进行了体积波等离子体低温内部灭菌的实验验证。下面，我们列出了本研究的主要结果。(1)氧等离子体杀菌机理的澄清：实验证实氧自由基的蚀刻效应是氧等离子体中主要的杀菌因素。在氧等离子体中没有观察到强紫外辐射，而在氮等离子体中，在290 ~ 400 nm范围内有几个强紫外辐射，表明紫外辐射是主要因素。(2)通过微波等离子体的开关控制来降低热效应：对于塑料医疗器械，由于材料不耐热，需要避免热效应。通过引入微波等离子体的时间调制操作，我们成功地将样品温度降低到70℃以下。(3)体波等离子体生产实验：为了对包裹物品内部进行灭菌，我们进行了体波等离子体灭菌实验。我们确认等离子体是在塑料瓶内产生的。采用脉冲微波等离子体对玻璃纸包裹的生物指示剂萎缩性芽孢杆菌（Bacillus Atrophaous）在20 min内进行灭菌。(4) OH自由基的影响：利用模拟空气的氮气-氧气混合气体，研究了加入水蒸气对微波等离子体杀菌性能的影响。结果表明，水蒸气对提高灭菌速度有重要作用。孢子细胞的崩溃可能与血浆中产生的OH自由基有关。(5)成果总结：发表期刊论文16篇，专著2部，国际会议论文集38篇，国内会议报告132篇。我们还申请了7项与现行等离子体灭菌相关的国内外专利，其中一项是等离子体灭菌方法专利。少

项目成果

期刊论文数量（94）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Gas barrier properties of hydrogenated amorphous carbon films coated on polymers by surface-wave plasma CVD

表面波等离子体CVD聚合物表面氢化非晶碳薄膜的阻气性能

DOI：
发表时间：
2007
期刊：
Thin Solid Films 515
影响因子：
0
作者：
H.Zhou;A.Ogino
通讯作者：
A.Ogino

マイクロ波プラズマの滅菌応用

微波等离子体灭菌应用

DOI：
发表时间：
2005
期刊：
化学工業 56・6
影响因子：
0
作者：
M.Nagatsu;T.Yoshida;M.Mesko;A.Ogino;T.Matsuda;T.Tanaka;H.Tatsuoka;K.Murakami;L.Xu;M.Siry;M.Siry;H.Nakano;T.Nagata;M.Nagatsu;L.Xu;M.Nagatsu;L.Xu;M.Nagatsu;H.Nonaka;A.Ogino;L.Xu;M.Siry;M.Nagatsu;M.Nagatsu;永津雅章
通讯作者：
永津雅章

Novel Plasma Sterilization Technique Using Volume Wave Plasma Excited by 2.45 GHz Microwaves

使用 2.45 GHz 微波激发体波等离子体的新型等离子体灭菌技术

DOI：
发表时间：
2005
期刊：
Abstracts and Full Papers CD of 17th Int'l.Symp. on Plasma Chemistry
影响因子：
0
作者：
L.Xu;P.Liu;R.J.Zhan;X.H.Wen;L.L.Ding;M.Nagatsu;M.Nagatsu;M.Nagatsu;M.Nagatsu;M.Nagatsu
通讯作者：
M.Nagatsu

Internal Sterilization Using Microwave-Excited Volume-Wave Plasma

使用微波激发体积波等离子体进行内部灭菌

DOI：
发表时间：
2004
期刊：
Proc.of International Symposium on Microwave Science and Its Application to Related Fields
影响因子：
0
作者：
L.Xu;P.Liu;R.J.Zhan;X.H.Wen;L.L.Ding;M.Nagatsu;M.Nagatsu;M.Nagatsu;M.Nagatsu;M.Nagatsu;L.Xu;M.Nagatsu;A.Ogino;M.Nagatsu;A.Ogino;L.Xu;H.Nonaka;M.Shido;T.Aoshi;M.Nagatsu;L.Xu;M.Nagatsu;L.Xu;A.Ogino;M.Nagatsu;M.Nagatsu;L.Xu;F.Terashita;H.Nonaka;M.Shido;M.Stefecka;M.Nagatsu;M.Nagatsu;L.Xu;F.Terashita
通讯作者：
F.Terashita

Diagnostics of surface-wave plasma with air-simulated N_2-O_2 gas mixture for low-temperature sterilization

空气模拟N_2-O_2混合气体低温灭菌表面波等离子体诊断