Investigation on protein adduct produced by alternatives to ozone-depleting substances and susceptibility genes and establishment of new method for risk assessment.

消耗臭氧层物质替代品与易感基因产生的蛋白质加合物的调查及风险评估新方法的建立。

基本信息

  • 批准号:
    16390169
  • 负责人:
  • 金额:
    $ 9.6万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2006
  • 项目状态:
    已结题

项目摘要

1. The neuronal effects of 1-bromopropane in two inbred rat strains WNA (Wistar Nagoya) and F344 were compared to find genes related with susceptibility to 1-bromopropane exposure. Oligonucleotide microarray was applied to detect the difference of gene expression in two strains. The results showed that F344 is more susceptible than WNA in neuronal effects. The microarray study showed that glutathione S-transferase and epoxy hydrolase's expression were enhanced thorough two strains. Neuronal regeneration related gene was enhanced in WNA but suppressed in F344, showing a contrasting effect on two strains. Further investigation is needed to understand how this gene is related with the difference of susceptibility in two strains.2. Metabolite N-acetyl S-propyl cysteine was detected with GC-MS and LC-MS/MS in the urine obtained from inhalation experiments using rats. This method is also applied to human samples successfully.3. Protein adduct S-propyl cysteine was detected with LC-MS/MS in globin obtained from rats exposed to 1-bromopropane. This method is applied to human samples successfully.4. Investigation on polymorphism of glutathione S-transferase clarify the dose responserelationship in the workers exposed to 1-bromopropane.
1.比较了1-溴丙烷对两个近交系大鼠品系WNA(Wistar名古屋)和F344的神经元效应,以寻找与1-溴丙烷暴露易感性相关的基因。应用寡核苷酸芯片检测两株菌基因表达的差异。结果表明,F344比WNA更易受到神经元效应的影响。基因芯片研究表明,谷胱甘肽S-转移酶和环氧水解酶的表达通过两个菌株增强。神经元再生相关基因在WNA中表达增强,而在F344中表达受到抑制,表现出对两个菌株的相反作用。该基因与两株易感性差异的关系尚需进一步研究.采用GC-MS和LC-MS/MS检测大鼠吸入实验所得尿液中的代谢产物N-乙酰基S-丙基半胱氨酸。该方法也成功地应用于人体样品.蛋白加合物S-丙基半胱氨酸检测与LC-MS/MS从大鼠接触1-溴丙烷获得的珠蛋白。该方法已成功应用于人体样品.谷胱甘肽S-转移酶多态性的研究阐明了接触1-溴丙烷工人谷胱甘肽S-转移酶的剂量反应关系。

项目成果

期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A survey on exposure level, health status, and biomarkers in workers exposed to 1-bromopropane
Investigation of Toxicity through Human Cases and Animal Experiments-study of bromopropanes-
通过人体案例和动物实验研究毒性-溴丙烷的研究-
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ryozo Matsuda;Tokuko Munesue (eds.);Ichihara S. et al.;Ichihara S. et al.;Ito Y.et al.;Okamura A. et al.;Ichihara G. et al.
  • 通讯作者:
    Ichihara G. et al.
A role for the aryl hydrocarbon receptor in regulation of ischemia-induced angiogenesis
A survey of semen indices in insecticide sprayers
  • DOI:
    10.1539/joh.46.109
  • 发表时间:
    2004-03-01
  • 期刊:
  • 影响因子:
    3
  • 作者:
    Kamijima, M;Hibi, H;Takeuchi, Y
  • 通讯作者:
    Takeuchi, Y
Pravastatin increases survival and suppresses an increase in myocardial matrix metalloproteinase activity in a rat model of heart failure
  • DOI:
    10.1016/j.cardiores.2005.08.001
  • 发表时间:
    2006-02-15
  • 期刊:
  • 影响因子:
    10.8
  • 作者:
    Ichihara, S;Noda, A;Yokota, M
  • 通讯作者:
    Yokota, M
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ICHIHARA Gaku其他文献

ICHIHARA Gaku的其他文献

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{{ truncateString('ICHIHARA Gaku', 18)}}的其他基金

Investigation on neurodegenerative effect of 1-bromopropane using new biomarkers
使用新生物标志物研究 1-溴丙烷的神经退行性作用
  • 批准号:
    24406018
  • 财政年份:
    2012
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Role of neurogenesis in mechanism of chronic or late-onset toxicity of environmental chemicals to the central nervous system
神经发生在环境化学物质对中枢神经系统慢性或迟发型毒性机制中的作用
  • 批准号:
    23659326
  • 财政年份:
    2011
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Study on oxidative stress and glial activation explaining demyelination in the central nervous system induced by alternative to freons
氧化应激和神经胶质激活研究解释氟利昂替代品引起的中枢神经系统脱髓鞘
  • 批准号:
    22390120
  • 财政年份:
    2010
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Investigation on toxicity of 1-bromopropane to central nervous system and hematopoetic function in humans
1-溴丙烷对人体中枢神经系统和造血功能的毒性研究
  • 批准号:
    21406017
  • 财政年份:
    2009
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of molecular and cellular targets in neurotoxicity of alternatives to ozone-depleting substances and establishment of biomarkers for the central nervous toxicity
分析臭氧消耗物质替代品神经毒性的分子和细胞靶标并建立中枢神经毒性生物标志物
  • 批准号:
    19390160
  • 财政年份:
    2007
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on dose-dependency of the central nervous system toxicity of 1-bromopropane in humans
1-溴丙烷对人体中枢神经系统毒性的剂量依赖性研究
  • 批准号:
    17406017
  • 财政年份:
    2005
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Investigation on neurologic abnormality induced by 1-bromoproane and individual variation in susceptibility
1-溴丙烷所致神经异常及个体易感性差异的研究
  • 批准号:
    14406015
  • 财政年份:
    2002
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
INVESTIGATION ON TOXICITIES OF ALTERNATIVES FOR CHLOROFLUOROCARBONS AND ITS RISK ASSESSMENT USING MOLECULAR AND BIOLOGICAL MARKERS.
氯氟碳替代品的毒性调查及其使用分子和生物标记物的风险评估。
  • 批准号:
    13470088
  • 财政年份:
    2001
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development Deregulating system for inhalation exposure to volatile organic chemicals (VOCs) for toxicity testing
开发用于毒性测试的挥发性有机化学品 (VOC) 吸入暴露放松管制系统
  • 批准号:
    12557039
  • 财政年份:
    2000
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study on mechanism of reproductive toxicity and neurotoxicity of 1-bromopropane, a new alternative to chlorofluorocarbons
氯氟烃新替代品1-溴丙烷生殖毒性和神经毒性机制研究
  • 批准号:
    11670367
  • 财政年份:
    1999
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Investigating protein adduct formation from exposure to perfluoroalkyl substances
研究暴露于全氟烷基物质中蛋白质加合物的形成
  • 批准号:
    516485-2017
  • 财政年份:
    2017
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Canadian Graduate Scholarships Foreign Study Supplements
Acetaldehyde Effect on Protein-Adduct Formation and GI Tract Substrate Transport
乙醛对蛋白质加合物形成和胃肠道底物运输的影响
  • 批准号:
    7275147
  • 财政年份:
    2007
  • 资助金额:
    $ 9.6万
  • 项目类别:
Acetaldehyde Effect on Protein-Adduct Formation and GI Tract Substrate Transport
乙醛对蛋白质加合物形成和胃肠道底物运输的影响
  • 批准号:
    7413587
  • 财政年份:
    2007
  • 资助金额:
    $ 9.6万
  • 项目类别:
Analysis of carcinogenic heterocyofic amine-protein adduct as environmental and biological marker
作为环境和生物标志物的致癌杂环胺蛋白加合物的分析
  • 批准号:
    12672171
  • 财政年份:
    2000
  • 资助金额:
    $ 9.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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