A basic study of gene and anti-angiogenic therapy for malignant brain tumor using bone marrow stromal cell and its potential of cell-fusion

1. 建立了大鼠和小鼠骨髓基质细胞的培养体系。FACS分析显示BMSCs表达CD34（-）、CD45（+/-）、CD90（+）和Sca1（+）。这些数据发表在Brain Res Protoc(2004).2。骨髓间充质干细胞移植到梗死脑后表现出活跃的细胞增殖。这些数据发表在Brain Res（2005）上。对培养的骨髓间充质干细胞进行了基因表达谱分析，结果表明骨髓间充质干细胞有可能在外界刺激下改变其基因表达模式。这些数据发表在Brain Res（2006）上。纤维蛋白胶可作为骨髓间充质干细胞移植到大鼠脑冷损伤模型或脊髓半断模型的支架材料。这些数据已准备好提交给“神经外科”或“神经创伤杂志”。我们考察了骨髓间充质干细胞与不同种类细胞的融合，以及在不同条件下骨髓间充质干细胞的体外分化情况。我们的免疫组织化学数据表明，骨髓间充质干细胞可能与神经元细胞融合，并在某些特定情况下表现出神经元分化。这些发现仍在用不同的方法进行调查。

1. We have established the culture system of the bone marrow stromal cells (BMSC) obtained from the rats and mice. FACS analysis showed BMSCs expressed CD34 (-), CD45 (+/-), CD90 (+) and Sca1 (+). These data were published in Brain Res Protoc (2004).2. The BMSCs presented active cell proliferation when it was transplanted into infarct brain. These data were published in Brain Res (2005).3. Gene expression profiling was performed for cultured BMSCs and it was clarified that the BMSCs had potentials to alter their gene expression patterns in response to external stimuli. The data were published in Brain Res (2006).4. Fibrin glue could be suitable biomaterial as a scaffold for the BMSCs when they were transplanted into brain cold injury model or spinal cord hemi-section model of the rats. These data has been prepared for submission to "Neurosurgery" or "Journal of Neurotrauma".5. We examined the cell-fusion between BMSCs and different kinds of cells, and differentiation of BMSC under various conditions in vitro. Our data from immunohistochemistry suggested that the BMSCs might be fused with neuronal cells and showed neuronal differentiation in some specific circumstances. These findings are still under investigating by different methods.