A genomic and phenomic investigation of a mitochondrial glutathione transferase

线粒体谷胱甘肽转移酶的基因组和表型组研究

• 批准号: DP0343815
• 负责人: Prof Philip Board
• 金额: $ 16.75万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0343815
• 关键词: genomic; phenomic; investigation; mitochondrial; glutathione

The aim of this study is to understand of the genomics, structure and function of glutathione transferase Kappa (GSTK), a novel GST found in mitochondria. The investigations will achieve several outcomes. (1)an understanding of the organisation of GSTK gene(s) in humans and mice; (2) determination of the role of GSTK in mitochondria, by investigating the phenotype of knockout mice; (3) determination of the crystal structure of human GSTK; (4) An understanding of GSTK's substrate specificity, reaction kinetics and structure/function relationships. Since GSTK is confined to mitochondria, and may not be related to other GSTs, we may also identify novel functions

本研究的目的是了解谷胱甘肽转移酶Kappa（GSTK）的基因组学、结构和功能，GSTK是一种在线粒体中发现的新型GST。调查将取得若干成果。 (1)an了解人类和小鼠GSTK基因的组织;（2）通过研究基因敲除小鼠的表型，确定GSTK在线粒体中的作用;（3）确定人GSTK的晶体结构;（4）了解GSTK的底物特异性、反应动力学和结构/功能关系。由于GSTK仅限于线粒体，并且可能与其他GST无关，因此我们还可以鉴定新的功能