Sympathetically mediated dental pain and its control

交感神经介导的牙痛及其控制

• 批准号: 17591984
• 负责人: SASANO Takashi
• 金额: $ 2.08万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591984/
• 关键词: Dental pain; Sympathetic nerve; Pulpitis; Pain control; axonal transport of WGA-HRP

1. Purpose Mastication needs cooperation of masticatory muscles, but the exact role of the each muscle for mastication is not completely clear. The purpose of this study was to evaluate the change of masticatory muscles under the unilateral masseter muscle dysfunction induced by Botulinum toxin A (BTXA) injection in rabbits. BTXA binds to presynaptic nerve endings and inhibits the release of acetylcholine, leading to temporary partial denervation and reduction of muscle fiber.2. Materials and Methods White Japanese rabbits were injected with 10 units of BTXA into the left masseter muscle. The effect of a neuromuscular blockade of masseteric activity was evaluated with serial computer tomography (CT) scans with 0.63mm slice thickness and 0.1mm slice interval taken after 4 weeks.3. Results The masseter muscle size of BTXA injected was reduced to about 80% against the control side. This indicated the morphologic change caused by the neuromuscular blockade.4. Coclusion To study the exact role of each muscles for mastication, masseter mucle dysfunction was made by BTXA injection into the masseter muscle. The most suitable dose, timing and the degree of dysfunction were established. The elrctro-myography is considered to investigate the functional change. This animal model helps the understanding of the mechanism of tempolo-mandibular dysfunction.

1.目的 咀嚼需要咀嚼肌的配合，但各块肌肉对于咀嚼的确切作用尚不完全清楚。本研究的目的是评价注射A型肉毒毒素（BTXA）引起的单侧咬肌功能障碍下咀嚼肌的变化。 BTXA与突触前神经末梢结合，抑制乙酰胆碱的释放，导致暂时性部分去神经支配和肌纤维减少。2．材料和方法日本白兔左咬肌注射10单位BTXA。神经肌肉阻断咬肌活动的效果通过4周后进行的连续计算机断层扫描(CT)扫描进行评估，扫描厚度为0.63mm，层间隔为0.1mm。 3．结果注射BTXA后咬肌大小较对照侧减少约80%。这表明神经肌肉阻滞引起的形态学变化。4．结论为了研究各块肌肉对于咀嚼的确切作用，通过将BTXA注射到咬肌中来造成咬肌功能障碍。确定了最合适的剂量、时间和功能障碍程度。肌电图被认为可以研究功能变化。该动物模型有助于理解颞下颌功能障碍的机制。

项目成果

Sympathetic never fibers sprout into rat odontoblast layer, but not into dentinal tubules, in response to cavity preparation

作为对空腔准备的反应，交感神经纤维发芽到大鼠成牙本质细胞层，但不发芽到牙本质小管

• 发表时间: 2008
• 期刊: Neutoscience Letter 435
• 作者: Shimeno Y;Sugawara Y;likubo M;Shoji N;Sasano T,
• 通讯作者: Sasano T,

Sympathetic nerve fibers in rat normal and inflamed dental pulp: absence from dentinal tubules

大鼠正常牙髓和发炎牙髓中的交感神经纤维：牙本质小管缺失

• 发表时间: 2008
• 期刊: Interface Oral Health Science 2007 2
• 作者: Y. Shimeno;Y. Sugawara;M. Iikubo;N.Shoji;T. Sasano
• 通讯作者: T. Sasano

Sympathetic nerve fibers sprout into rat odontoblast layer, but not into dentinal tubules, in response to cavity preparation.

作为对空腔准备的反应，交感神经纤维发芽进入大鼠成牙本质细胞层，但不进入牙本质小管。

• 发表时间: 2008
• 期刊: Neuroscience Letters 435
• 作者: Yoichi Shimeno;Yumiko Sugawara;Masahiro Iikubo;Noriaki Shoji;Takashi Sasano
• 通讯作者: Takashi Sasano

「研究成果報告書概要(和文)」より

摘自《研究结果报告摘要（日文）》

• 发表时间: 2005
• 作者: Kawauchi;et. al.;Nishimura et al.;Dezawa et al.;Yoshizawa et al.;星野 幹雄;星野 幹雄
• 通讯作者: 星野 幹雄