Mechanism of inhibitory activity of lenthionine against platelet aggregation and its ex vivo effect

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Inhibiroy activity of lenthionine against signal transduction in plateletsInhibitory activity of lenthionine, the most abundant shiitake flavor component, atainst platelet aggregation was examined. Lenthionine inhibited platelet aggregation induced by arachidonic acid and U-46619. The inhibitory activity of authentic lenthionine was almost similar to that of lenthionine in the essential oil from shiitake, indicating that lenthionine was the most active component in the essential oil.Then, platelet was activated by using various agonists such as A23187, PMA, PAF, and ADP, to examine the inhibitory mechanizm of lenthionine. Lenthionine inhibited the aggreation of plateles induced by every agonists tested. This result indicates that lenthionie would not inhibit signal transduction cascade in platelets but inhibit adhesion and shape change of platelets, receptors for platelet activation, or phosphorylation of proteins.Inhibition of ex vivo platelet aggregation by lenthionineLenthionine was orally administered to rats and blood was withdrawn after a certain interval to examine its ex vivo effect on platelet aggregation. Oral-administrated lenthionine significantly inhibited platelet aggregation 8-20 hours after the administration. Then, dose of lenthionine was varied to examine the minimum dose to exert the inhibitory activity against platelet aggregation. As a result, more than 1 mg/kg of lenthionine significantly inhibited platelet aggregation.