Mechanism of inhibitory activity of lenthionine against platelet aggregation and its ex vivo effect

绵硫氨酸抑制血小板聚集的机制及其离体作用

• 批准号: 18580130
• 负责人: KUMAGAI Hitomi
• 金额: $ 2.49万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580130/
• 关键词: Ienthionine; shiitake mushroom; platelet aggregation; cyclic sulfuric compound; ex vivo experiment; 膜タンパク質GPIIb; IIIa; フィブリノーゲン; アグレゴメーター; フローサイトメーター

Inhibiroy activity of lenthionine against signal transduction in plateletsInhibitory activity of lenthionine, the most abundant shiitake flavor component, atainst platelet aggregation was examined. Lenthionine inhibited platelet aggregation induced by arachidonic acid and U-46619. The inhibitory activity of authentic lenthionine was almost similar to that of lenthionine in the essential oil from shiitake, indicating that lenthionine was the most active component in the essential oil.Then, platelet was activated by using various agonists such as A23187, PMA, PAF, and ADP, to examine the inhibitory mechanizm of lenthionine. Lenthionine inhibited the aggreation of plateles induced by every agonists tested. This result indicates that lenthionie would not inhibit signal transduction cascade in platelets but inhibit adhesion and shape change of platelets, receptors for platelet activation, or phosphorylation of proteins.Inhibition of ex vivo platelet aggregation by lenthionineLenthionine was orally administered to rats and blood was withdrawn after a certain interval to examine its ex vivo effect on platelet aggregation. Oral-administrated lenthionine significantly inhibited platelet aggregation 8-20 hours after the administration. Then, dose of lenthionine was varied to examine the minimum dose to exert the inhibitory activity against platelet aggregation. As a result, more than 1 mg/kg of lenthionine significantly inhibited platelet aggregation.

香菇中含量最高的香菇风味成分香菇素对血小板聚集的抑制活性。Lenthionine抑制花生四烯酸和U-46619诱导的血小板聚集。结果表明，香菇挥发油中的活性成分为香菇乙素，香菇乙素的抑制活性与香菇挥发油中的香菇乙素相近，说明香菇乙素是香菇挥发油中活性最强的成分。来噻鲁肽对各受体激动剂诱导的血小板聚集均有抑制作用。这一结果表明，lenthionie不会抑制血小板中的信号传导级联，但会抑制血小板的粘附和形状改变、血小板活化受体或蛋白质的磷酸化。lenthionineLenthionie对离体血小板聚集的抑制作用口服给予大鼠，并在一定间隔后采血，以检查其对血小板聚集的离体作用。在给药后8-20小时，口服给药的仑噻鲁肽显著抑制血小板聚集。然后，改变仑噻鲁肽的剂量以检查发挥抗血小板聚集的抑制活性的最小剂量。结果，大于1 mg/kg的乐噻鲁肽显著抑制血小板聚集。

项目成果

レンチオニンの血小板凝集抑制作用について

关于香菇素的血小板聚集抑制作用

• 发表时间: 2007
• 作者: 島田 正一郎;熊谷 日登美;桜井 英敏
• 通讯作者: 桜井 英敏

レンチオニンの血小板活性化に及ぼす影響

香菇多糖对血小板活化的影响

• 发表时间: 2006
• 作者: 島田 正一郎;熊谷 一樹;佐藤 由佳;熊谷 日登美;桜井 英敏
• 通讯作者: 桜井 英敏

Inhibition of Platelet Aggregation by Orally-Administered Lenthionine, a Key Flavor Compound in Shiitake Mushrooms (Lentinula edodes)

口服香菇（Lentinula edodes）中的一种主要风味化合物——伦硫氨酸对血小板聚集的抑制作用

• 发表时间: 2008
• 期刊: J.Clin.Biochem.Nutr. 43巻
• 作者: Shimada S.;Kumagai H.;Akao M.;Sakurai H.
• 通讯作者: Sakurai H.

Inhibition of ex vivo Platelet Aggregation by Lenthionine,a Key Flavor Compound in the Shiitake Mushroom,and Its Inhibitory Mechanis

香菇中关键风味物质绵硫氨酸对离体血小板聚集的抑制及其抑制机制

• 发表时间: 2007
• 作者: Shoichiro Shimada;Hidetoshi Sakurai;Hitomi Kumagai
• 通讯作者: Hitomi Kumagai

Inhibition of ex vivo Platelet Aggregation by Lenthionine, a Key Flavor Compound in the Shiitake Mushroom, and Its Inhibitory Mechanism

香菇中关键风味物质绵硫氨酸对离体血小板聚集的抑制及其抑制机制

• 发表时间: 2007
• 作者: Shoichiro;Shimada;Hidetoshi;Sakurai;Hitomi;Kumagai
• 通讯作者: Kumagai