Study on the formation and function of exosomes

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Exosomes are composed of proteins and lipids, which are secreted from cells including those infected with bacteria. In order to examine the structure of lipid A portion of LPS, which is potentially involved in the exosomes, we developed a method for mass spectrometry analysis of lipid A. We found that 2, 5-dihydroxybenzoic acid matrix was preferable for the detection of aminoarabinose modification. Salmonella Typhimurium LpxR potentially detoxifies lipid A by 3'-O-deacylation ; however, the involvement of deacylation in its adaptation to host cells remains unclear. Intracellular replication assay demonstrated that growth of the lpxR-null strain was lower than that of the wild-type strain, indicating that lipid A 3'-O-deacylation is beneficial for intracellular growth.