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The possible implication of fetal alcohol exposure on the behavioral abnormalities in the young adult.

胎儿酒精暴露对年轻人行为异常的可能影响。

基本信息

批准号：
18390322
负责人：
SAITO Toshikazu
金额：
$ 11.19万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390322/
关键词：
brain nerve neuroscience regenerative medicine neural stem cell fetal alcohol syndrome neural network 小胞体ストレス神経新生 NRSF REST

项目摘要

In the present study, we investigated the possible implication of prenatal alcohol exposure on the behavioral abnormalities in the post natal young period, and analyzed the possibility of neural stem cell (NSC) transplantation as a new therapy for abnormal behaviors of young/adult, using fetal alcohol syndrome (FAS) model animal. First, we transplanted marked NSCs to the control and fetal alcohol spectrum disorder (FASD) model rats intravenously, and evaluated the behavioral change and localization/dynamics of transplanted NSCs in the brain. The marked NSCs with [35S]methionine have been shown to accumulate in various brain regions in the FASD model rats rather than the brains of control animals. It was also indicated that the transplanted NSCs were localized in the areas such as cortex, striatum, hippocampus, SVZ, by the method of marking of NSCs by fluorescence dye. We also developed the newly method for the effective migration of intravenous transplanted NSCs into brain by using specific gel material which protects cells against the immune attack or enzymatic degradations in the body circulation. Additionally, we investigated the induction of behavioral change of FASD model rat by NSC transplantation in the context of anxiety, cognitive function, activity, and indicated the behavioral recovery of the model rats to the levels of control rats.

本研究利用胎儿酒精综合征(Fas)模型动物，研究了产前酒精暴露对幼年后行为异常的可能影响，并分析了神经干细胞(NSC)移植作为治疗青壮年异常行为的新方法的可能性。首先，我们将标记的NSCs静脉移植到对照组和胎儿酒精谱障碍(FASD)模型大鼠，并观察移植的NSCs在脑内的行为变化和定位/动力学。用[35S]蛋氨酸标记的神经干细胞在FASD模型大鼠的不同脑区积聚，而不是在对照组动物的大脑中。用荧光染料标记NSCs的方法表明，移植的NSCs定位于皮质、纹状体、海马区、SVZ等区域。我们还开发了一种新的方法，通过使用特定的凝胶材料来保护细胞免受体内循环中的免疫攻击或酶降解，从而有效地将静脉移植的神经干细胞迁移到脑内。此外，我们还从焦虑、认知功能、活动等方面考察了神经干细胞移植对FASD模型大鼠行为的影响，并表明模型大鼠的行为恢复到了对照组的水平。