Development of Thin Resin Film-BMP Composite

本项目的目的是寻找一种方法，通过使用具有与BMP和/或宿主来源细胞形成上述相同形状的能力的支架，然后植入活组织中，在体内诱导新骨，其充分适应骨缺损区域的形状。本课题成功地制备了具有诱导成骨作用的EMA树脂-BMP复合材料，并对树脂材料形成的骨形态进行了三维检测，利用GST标记基因建立了材料细胞毒性的快速筛选系统。在含有PLGA的新创新树脂的表面上，结合BMP。该系统显示了稳定的新骨形成，并且载体如PLGA作为BMP的载体显示出优异的结果，并且还证明了其能够替代聚乳酸的糊精。此外，在世界范围内第一次通过高速、X-射线衍射仪， ...更多信息 X线CT扫描，并显示了本实验的主要研究对象--BMP-树脂膜新骨形成的三维图像。利用荧光素酶基因与GST共转染培养细胞来检测细胞毒性，建立自发光生物检测体系是一项引人注目的成果。然而，在EMA树脂上培养细胞并不能获得良好的效果。用本组常用的未分化骨骼肌器官培养系统进行同样的培养。最终，该系统也显示出组织的不良聚集。尽管如此，这些实验所显示的信息对于未来的工作是极其重要的，并且EMA树脂系统中可能含有的潜在分子可能导致间充质组织增殖的抑制。通过该项目获得的系统，如BMP三维支架，将有助于开发新的组织工程方法，该项目将从现在开始不断进行实验。减

The objective of this project is to seek for the method to induce new bone in vivo which is adequately adapted to the shape of bone defect area by using scaffolds which has capability to form the same shape as mentioned as above with BMP and/or host origin cells, then implanted into the living tissue. The project has succeeded to make the EMA resin-BMP composites which induce new bone, three dimensional detection of the shape which was formed by resin material and rapid screening system for the cytotoxicity of materials by using the marker gene of GST. On the surface of the newly innovated resin that is containing PLGA, BMP was combined. This system revealed the stable new bone formation, and carrier such as PLGA showed excellent result as carrier of BMP, and it was also proven to be able to substitute the dextrin for the polylactic acid. Moreover, three dimensions of the bone induction and changes the passing time were clarified for the first time in the world wide by a high-speed, X- … More ray CT scan and also showed the 3-D image of new bone formation by thin resin film with BMP which is the main object of this experiment. Establishment of the bioassay system of self-luminescence using luciferase gene co-transfected with GST into the cultured cells to detect the cytotoxyty is remarkable achievement. However, to cultivate cells on the EMA resin could not have good result. Same kind of cultivation was performed by the organ culture system of undifferentiated skeletal muscle, which has been commonly experimented in this group. Eventually, this system also showed poor aggregation of tissues. Nonetheless, the information of these experiments showing was extremely important for the future work and the possible potential molecules containing in the EMA resin system may cause the inhibition of the mesenchymal tissue proliferation. Systems obtained through this project, such as 3 dimensional scaffolds with BMP, will attribute to developing new tissue engineering methods, and this project will be continuously experimented from now on. Less