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Analysis of development, differentiation, and remodeling of the pelvic visceral motor nervous system

盆腔内脏运动神经系统的发育、分化和重塑分析

基本信息

批准号：
18500267
负责人：
FUNAKOSHI Kengo
金额：
$ 2.59万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

In order to investigate the development and differentiation of the lumbosacral motoneuronal groups including the pelvic visceral motoneurons, we examined the expression of LIM proteins, Islet-1 and Islet-2, which are assumed to play a role in inducing motoneuronal fate. Islet-1 was expressed at E13.5 in the somatic motoneuronal groups and in the intermediolateral nucleus consisting of the parasympathetic preganglionic neurons, but was downregulated remarkably in the motoneuronal groups, except for the caudal level of the dorsolateral nucleus, which are homologous to the nucleus of Onuf. Therefore, Onuf's nucleus has a chemical identity different from any other somatic motoneuronal groups in the developmental process. Furthermore, differential spatiotemporal Islet-1 expression pattern among somatic motoneuronal groups led us to suppose that Islet-1 might be concerned with not only inducing motoneuronal fate as already shown, but also with the differentiation of motoneuronal groups. On the other hand, we also examine the primary sensory projections to the spinal cord using TRPV1 and CGRP as markers in order to investigate the development of the reflex pathway regarding pelvic visceral nerves, and elucidated that the reflexive pathways via direct projections of the primary sensory neurons to the parasympathetic preganglionic neurons were established by E16.In the phylogenic aspect, the general organization of the sympathetic preganglionic neurons in Xenopus was thoroughly examined. The sympathetic preganglionic neurons were divided mediolaterally into four distinct columns, and most of the neurons were located in the medially located intercalated nucleus, and in the laterally located intermediolateral nucleus. Furthermore, neurons concerned with the regulation of the abdominal viscera including the pelvic organs via the splanchnic nerves were clustered in the intermediolateral columns, unlike in mammals.

为了研究腰骶部运动神经元群（包括盆腔内脏运动神经元）的发育和分化，我们研究了LIM蛋白Islet-1和Islet-2的表达，这些蛋白被认为在诱导运动神经元命运中起作用。Islet-1在E13.5在躯体运动神经元组和由副交感节前神经元组成的中间外侧核中表达，但在运动神经元组中显著下调，除了与Onuf核同源的背外侧核的尾侧水平。因此，Onuf核在发育过程中具有不同于任何其他体细胞运动神经元群的化学特性。此外，体细胞运动神经元组之间的差异时空胰岛-1的表达模式使我们假设，胰岛-1可能不仅与诱导运动神经元的命运，如已经显示的，但也与分化的运动神经元组。另一方面，我们还以TRPV 1和CGRP为标记物，研究了初级感觉神经元向脊髓的投射，以探讨盆内脏神经反射通路的发育，并阐明了初级感觉神经元向副交感节前神经元直接投射的反射通路是在E16建立的。彻底检查了爪蟾中交感神经节前神经元的一般组织。交感神经节前神经元可分为4列，主要分布于中间外侧核和中间内侧核。此外，与哺乳动物不同的是，与内脏神经调节腹部内脏包括盆腔器官有关的神经元聚集在中间外侧柱中。