New Methods for Structural Biology in Solution

溶液中结构生物学的新方法

• 批准号: DP0342445
• 负责人: Prof Gottfried Otting
• 金额: $ 86.91万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0342445
• 关键词: New; Methods; Structural; Biology; Solution

New technologies will be developed that are sufficiently rapid and inexpensive to compete with and replace the mutagenesis experiments that biologists usually perform to identify and characterize the functionally important parts of a protein. Nuclear magnetic resonance (NMR) spectroscopy techniques in combination with various selective labelling schemes will be developed with the goal of identification and structural characterization of protein-ligand interactions at increased rates and enhanced accuracy. In addition, the three-dimensional structures of proteins and protein domains of biologically important functions and unknown fold will be determined by NMR. The project aims at techniques of direct impact in pharmaceutical industry.

将开发出足够快速和廉价的新技术，以与生物学家通常进行的诱变实验竞争并取代诱变实验，以识别和表征蛋白质的功能重要部分。核磁共振（NMR）光谱技术结合各种选择性标记计划将开发与蛋白质-配体相互作用的识别和结构表征的目标，在增加的速度和提高的准确性。此外，蛋白质和蛋白质结构域的生物学重要功能和未知折叠的三维结构将通过NMR确定。该项目旨在研究对制药工业有直接影响的技术。