New methods for structural biology and drug discovery by nuclear magnetic resonance spectroscopy

核磁共振波谱技术用于结构生物学和药物发现的新方法

• 批准号: DP140100304
• 负责人: Prof Gottfried Otting
• 金额: $ 48.58万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140100304
• 关键词: New; methods; structural; biology; drug

Paramagnetic lanthanide tags offer fresh opportunities in structural biology and for rational drug design. Novel nuclear magnetic resonance (NMR) spectroscopy techniques will selectively detect the NMR signals from protein regions marked by paramagnetic lanthanides, accelerating the structure analysis of protein-ligand complexes. New lanthanide tags will bind to phosphoserine and selenocysteine residues site-specifically introduced into proteins. These tags will also enable accurate distance measurements by electron paramagnetic resonance (EPR) spectroscopy in large, biologically important protein systems hitherto not amenable to detailed structural studies and in proteins undergoing conformational changes.

顺磁性镧系标签为结构生物学和合理的药物设计提供了新的机会。新型的核磁共振（NMR）波谱技术将选择性地检测顺磁性镧系元素标记的蛋白质区域的核磁共振信号，加速蛋白质配体复合物的结构分析。新的镧系标签将结合磷酸丝氨酸和硒代半胱氨酸残基特异性引入蛋白质。这些标签还可以通过电子顺磁共振（EPR）光谱在大型、生物学上重要的蛋白质系统中进行精确的距离测量，迄今为止还不能进行详细的结构研究，也不能进行构象变化的蛋白质。