Ultrafast magic angle spinning solid-state nuclear magnetic resonance capability

超快魔角旋转固态核磁共振能力

• 批准号: LE180100026
• 负责人: Prof Gottfried Otting
• 金额: $ 11.92万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2018
• 资助国家: 澳大利亚
• 起止时间: 2018-04-12 至 2020-12-21
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE180100026
• 关键词: Ultrafast; magic; angle; spinning; solid

This project aims to extend an existing nuclear magnetic resonance (NMR) spectrometer for structural investigations of proteins in the solid state. Many proteins, such as amyloids and flexible proteins, cannot be studied by X-ray crystallography, solution NMR spectroscopy or cryoelectron microscopy, because they cannot be crystallised or are not sufficiently soluble, or are structurally too heterogeneous. This project will extend the capability of an existing 800 MHz NMR spectrometer to solid-state NMR. By offering ultrafast magic angle spinning speeds, the system aims to afford greatly enhanced sensitivity and multidimensional NMR spectra of protein systems not previously amenable to structural analysis by NMR spectroscopy or other techniques. This will have important applications in biotechnology and biomedicine.

该项目旨在扩展现有的核磁共振（NMR）光谱仪，用于固态蛋白质的结构研究。许多蛋白质，如淀粉样蛋白和柔性蛋白，不能通过X射线晶体学，溶液NMR光谱学或冷冻电子显微镜进行研究，因为它们不能结晶或不充分溶解，或者结构太不均匀。该项目将把现有的800 MHz核磁共振波谱仪的能力扩展到固态核磁共振。通过提供超快魔角旋转速度，该系统旨在提供大大增强的灵敏度和蛋白质系统的多维NMR光谱，这些蛋白质系统以前不适合通过NMR光谱或其他技术进行结构分析。这将在生物技术和生物医学中有重要的应用。