Fluorine-labelled proteins for NMR spectroscopy

用于 NMR 光谱的氟标记蛋白质

• 批准号: DP0664184
• 负责人: Prof Gottfried Otting
• 金额: $ 26.86万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2006
• 资助国家: 澳大利亚
• 起止时间: 2006-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0664184
• 关键词: Fluorine; labelled; proteins; NMR; spectroscopy

The technique developed in this project has direct impact on pharmaceutical research: NMR spectroscopy is used routinely to identify chemical compounds that bind to protein targets. This project includes the development of novel assignment techniques of 19F-labelled proteins, so that 19F-NMR can be used to detect specific binding interactions. One of the methods proposed here is designed to reveal structural information about the binding mode in solution with atomic detail. This knowledge can significantly accelerate drug development. It is otherwise only available from crystal structures that can not always be determined.

该项目开发的技术对药物研究有直接影响：NMR光谱法通常用于识别与蛋白质靶点结合的化合物。该项目包括开发19 F标记蛋白质的新分配技术，以便19 F-NMR可用于检测特异性结合相互作用。这里提出的方法之一是旨在揭示与原子细节的解决方案中的结合模式的结构信息。这些知识可以大大加速药物开发。否则，它只能从晶体结构中获得，而晶体结构并不总是可以确定的。