Experimental study for fusion vaccine of dendritic cell/ stromal cell/tumor cell transfected with LIGHT

LIGHT转染树突状细胞/基质细胞/肿瘤细胞融合疫苗的实验研究

• 批准号: 18790960
• 负责人: NAKAMURA Tetsu
• 金额: $ 1.75万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2008
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18790960/
• 关键词: LIGHT; 癌ワクチン; LTβR; 樹状細胞; 腫瘍細胞

マウス腫瘍細胞の細胞膜上におけるLIGHTの発現は、今回の検討では困難であったが、COS7細胞におけるLIGHT発現の検討では細胞質内での発現は認めた。原因としてC末端におけるFLAG修飾が起因している可能性があり今後発現ベクターの改良により細胞膜上に機能的なLIGHT発現させることが可能と考える。

The LIGHT was detected on the cell membrane. This time, it was found that the cell was trapped, and the COS7 cell was detected in the cell. The LIGHT was detected in the cell. The reason is that the C-terminal FLAG repair is not possible. In the future, it is possible to improve the mechanism on the cell membrane by means of LIGHT.

项目成果

Correlation between expression of major histocompatibility complex class I and that of antigen presenting machineries in carcinoma cell lines of the pancreas, biliary tract and colon.

胰腺、胆道和结肠癌细胞系中主要组织相容性复合物 I 类的表达与抗原呈递机制之间的相关性。

• 发表时间: 2006
• 期刊: Kobe Journal of Medical Science 52・3-4
• 作者: Yoshiyasu;Mita;Yasuda T;Yasuda T;Koyama T;Ajiki T;Imanishi T
• 通讯作者: Imanishi T

Superior anti-tumor protection and therapeutic efficacy of vaccination with allogeneic and semiallogeneic dendritic cell/tumor cell fusion hybrids for murine colon adenocarcinoma

• DOI: 10.1007/s00262-006-0252-5
• 发表时间: 2007-07-01
• 期刊: CANCER IMMUNOLOGY IMMUNOTHERAPY
• 影响因子: 5.8
• 作者: Yasuda, Takashi;Kamigaki, Takashi;Kuroda, Yoshikazu
• 通讯作者: Kuroda, Yoshikazu

The anti-tumor efficacy by regulatory T cells depletion in addition to DC vaccines for murine pancreatic cancer

除 DC 疫苗外，消除调节性 T 细胞对小鼠胰腺癌的抗肿瘤功效

• 发表时间: 2008
• 作者: M Yamamoto;T Kamigaki;Y Iwatani;T Nakamura;Y Kuroda
• 通讯作者: Y Kuroda