Development of surface modification method of titanium implant for rapid bone-bonding

作者发现，用10- 20 mmol/L氯化钙水溶液在200℃水热处理钛种植体，在种植体表面形成含少量钙的氧化钛膜，促进类骨磷灰石的生成和成骨细胞的初始附着和增殖。将在10- 20 mmol/L氯化钙水溶液中进行水热处理的钛种植体植入大鼠胫骨，观察种植体与骨的结合情况，结果显示骨结合率为55%在植入1、2和4周后，分别有86%（未处理为5.7%）、86%（未处理为19.2%）和96%（未处理为57%）的高值。种植体与骨之间无结缔组织介入，直接接触率高。为检测钛种植体与骨的结合强度，将水热处理后的钛种植体试件在氯化钙溶液中植入大鼠胫骨，进行拔出试验。氯化钙水热处理钛种植体1周和4周后的结合强度分别为1 N/mm^2和1.7N/mm^2，1周后的强度与4周后的强度相当，有望成为划时代的表面改性方法。具有“在早期阶段的微移动阻力”、“在早期阶段的负载可能性”、“愈合期显著缩短的可能性”和“高结合强度的表现”。

The authors found that the titanium oxide film which contained small amount Ca in the implant surface was formed by hydrothermal-treating a titanium implant with 10-20mmol/L calcium chloride aqueous solution at 200℃ and then that the generation of the bone-like apatite and initial attachment and proliferation of osteoblast cells were promoted. Moreover, the titanium implant which hydrothermal-treated in the calcium chloride aqueous solution of 10-20mmol/L was implanted in the tibia of the rat, and the connection condition of the implant and the bone was examined.It was shown that the bone-contact ratio was 55% (to be non-treated was 5.7%), 86% (to be non-treated was 19.2%), and 96% (to be non-treated was 57%) of high values after 1, 2, and 4 weeks implantation, respectively. The connective tissue did not intervene between a bone and an implant, and the direct-contact existed at the high rate. Therefore, it was suggested that this treatment was the method which obtained high bond-strength from the early stages after implantation.To examine the bonding strength between the titanium implant and the bone, the hydrothermal-treated implant specimen in calcium chloride solution was implanted in the tibia of the rat and pulling-out test was carried out. The bonding strength of hydrothermal-treatment titanium implant using calcium chloride solution indicated 1N/mm^2 and 1.7N/mm^2 for 1 and 4 weeks after implantation, respectively, and it became clear that the strength of hydrothermal-treated specimen after 1 week was equal to that of non-treated specimen after 4 weeks.This treatment could expect to be the epoch-making surface modification method, with "the resistance of micro-movement at the early stage", "possibility of the load at the early stage", "possibility of the substantial shortening of the healing-up period", and "expression of a high bonding-strength".