Molecular Design of Oxygenases Applicable to Synthetic Chemistry

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The study has achieved creation of novel artificial oxygenases founded on a heme protein deriving from a thermophile or a cytochrome P450 protein that utilizes H2O2 as the oxidant. A newly developed method,"Decoy molecule" succeeded to rationally modify the active site of the P450 protein in addition to conventional mutagenesis techniques. The artificial oxygenases exerted high catalytic activity and/or thermal tolerance which were not achieved by ever reported artificial oxygenases. Results in the study provide ideas and methodology to design artificial oxygenases aiming practical uses and might be applicable to artificial enzymes of other activities.