Analysis of proliferation and differentiation of human embryonic stem cells and research for clinical application

点击翻译按钮获取中文摘要

We attempt to discover the culture system for generation of desired lineage-specific stem cells from human ES cells and succeeded in generating progenitor cells for hematopoietic cells, endothelial cells, skeletal muscle, cardiomyocytes and neural cells.VEGFR-2(+)CD34(+) cells are identified as a candidate of primate hemangioblasts. We established a novel protocol to derive myogenic precursors from murine ES/iPS cells with a monoclonal antibody SM/C-2.6 that recognizes quiescent satellite cells. SM/C-2.6-positive cells are highly myogenic and efficiently differentiate into myofibers both in vitro and in vivo. We recently developed culture system for skeletal muscle cells from human ES cells.Recently, we developed an excellent humanized model mouse, NOG mouse, in which successful engraftment was achieved even if small number of CD34^+ cells were transplanted into the mice. In this study we confirmed that NOG mouse was useful for evaluation of ES/iPS cell-derived tissue specific stem cells.