Search of the gene conferring tannin accumulation in persimmon fruit and its utilization for the analysis of genome composition and the seedling selection

柿果实单宁积累基因的寻找及其在基因组组成分析和幼苗选择中的应用

• 批准号: 19380019
• 负责人: YONEMORI Keizo
• 金额: $ 13.15万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380019/
• 关键词: マメガキ; 倍数体; 甘渋性決定遺伝子; 転写因子; 定量PCR; DNAマーカー; 遺伝子単離; プロアントシアニジン; AFLP; 羅田甜柿; 甘渋性関与遺伝子; 遺伝子発現; フラボノイド合成; タンニン細胞

The gene conferring the trait of astringency-loss in Japanese-type PCNA is shown to be located at 100kb away of fosmid contig constructed from Diospyros lotus, a close relative of D.kaki. This single gene controls the astringency trait in persimmon fruit and 6 alleles are responsible for the appearance of this trait. The DkMyb4, a regulatory gene, is also found to control tannin biosynthesis in the fruit and is indicated to determine whether an individual is PCNA or non-PCNA due to its strength of expression. Using the DNA sequences linked to this gene, we could construct a primer pair for PCR analysis for selecting PCNA offspring of Japanese-type. In addition, we also found an AFLP marker to distinguish Chinese-type PCNA among breeding population.

日本型PCNA中赋予涩味丧失性状的基因被证明位于离Diospyros lotus构建的Fosmid重叠群100kb处，Diospyros lotus是D.kaki的近亲。该基因控制柿果实的涩味性状，有6个等位基因负责该性状的出现。DkMyb 4是一种调控基因，也被发现控制果实中单宁的生物合成，并由于其表达强度而被指示用于确定个体是PCNA还是非PCNA。利用与该基因连锁的DNA序列，我们可以构建一对用于PCR分析的引物，以选择日本型PCNA后代。此外，我们还发现了一个AFLP标记，以区分中国型PCNA的育种群体。

项目成果

Identification of the genetic region including astringency (AST) locus in hexaploid persimmon (Diospyros kaki Thunb.) by positional cloning in a diploid relative. D. lotus

通过二倍体近缘种的位置克隆鉴定六倍体柿子（Diospyros kaki Thunb.）中包括涩味（AST）位点的遗传区域。

• 发表时间: 2009
• 作者: T.Tsujimoto;T.Akagi;A.Kono;N.Mitani;K.Yonemori
• 通讯作者: K.Yonemori

Quantitative real-time PCR to determine allele number for the astringency locus by analysis of a linked marker in Diospyros kaki Thunb

• DOI: 10.1007/s11295-009-0202-4
• 发表时间: 2009-07-01
• 期刊: TREE GENETICS & GENOMES
• 影响因子: 2.4
• 作者: Akagi, Takashi;Kanzaki, Shinya;Yonemori, Keizo
• 通讯作者: Yonemori, Keizo

Identification of the genetic region including astringency (AST) locus in hexaploid persimmon (Diospyros kaki Thunb. ) by positional cloning in a diploid relative, D. lotus

通过在二倍体近缘种 D. Lotus 中定位克隆鉴定六倍体柿子 (Diospyros kaki Thunb.) 中包括涩味 (AST) 位点的遗传区域

• 发表时间: 2010
• 作者: T. Tsujimoto;T. Akagi;A. Kono;N. Mitani;K. Yonemori
• 通讯作者: K. Yonemori

DkMyb4 Is a Myb Transcription Factor Involved in Proanthocyanidin Biosynthesis in Persimmon Fruit

• DOI: 10.1104/pp.109.146985
• 发表时间: 2009-12-01
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Akagi, Takashi;Ikegami, Ayako;Yonemori, Keizo
• 通讯作者: Yonemori, Keizo

SCAR Markers for Practical Application of Marker-assisted Selection in Persimmon (Diospyros kaki Thunb.) Breeding

• DOI: 10.2503/jjshs1.79.150
• 发表时间: 2010-04
• 期刊: Journal of The Japanese Society for Horticultural Science
• 作者: S. Kanzaki;T. Akagi;T. Masuko;M. Kimura;M. Yamada;A. Sato;N. Mitani;Naoki Ustunomiya;K. Yonemori-K.-Y