Search of the gene conferring tannin accumulation in persimmon fruit and its utilization for the analysis of genome composition and the seedling selection

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The gene conferring the trait of astringency-loss in Japanese-type PCNA is shown to be located at 100kb away of fosmid contig constructed from Diospyros lotus, a close relative of D.kaki. This single gene controls the astringency trait in persimmon fruit and 6 alleles are responsible for the appearance of this trait. The DkMyb4, a regulatory gene, is also found to control tannin biosynthesis in the fruit and is indicated to determine whether an individual is PCNA or non-PCNA due to its strength of expression. Using the DNA sequences linked to this gene, we could construct a primer pair for PCR analysis for selecting PCNA offspring of Japanese-type. In addition, we also found an AFLP marker to distinguish Chinese-type PCNA among breeding population.