Isolation of the gene conferred astringency in persimmon and its functional analysis for the mechanisms on tannin accumulation.

柿子涩味基因的分离及其单宁积累机制的功能分析。

• 批准号: 22380023
• 负责人: YONEMORI Keizo
• 金额: $ 12.31万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380023/
• 关键词: 園芸学; プロアントシアニジン; タンニン; ゲノム; 遺伝子; 植物

Fosmid library was constructed by a diploid relative, Diospyros lotus, of D. kaki (hexaploid) for isolation of AST gene which confers astringency trait in persimmon. After screening seed clone from fosmid library by using a probe of AST-linked marker, fosmid contig was developed by further screening of the library using end-sequences of the clone. In addition, BAC library of D. lotus was constructed and BAC contig was also developed. Each contig seemed to contain AST locus there, so that we were approaching very near to isolate the AST gene. Furthermore, this BAC library was shown to be effective to isolate the CPCNA gene which confers the trait of non-astringency in Chinese-type non-astringent persimmon. Using the BAC library, isolation of CPCNA gene may also be possible.

利用柿的二倍体亲缘种柿(Diospyros Lotus)(六倍体)构建了一个用于分离柿收敛性状AST基因的Fosid文库。用AST连锁标记的探针从Fosmid库中筛选种子克隆后，利用克隆的末端序列进一步筛选文库，得到FosmidContig。此外，还构建了莲花的BAC文库，并开发了BAC重叠群。每个重叠群似乎都含有AST基因，所以我们离分离AST基因很近了。此外，该BAC文库能够有效地分离出具有不收敛特性的中式不涩柿CPCNA基因。利用BAC文库也有可能分离出CPCNA基因。

项目成果

Diploid/hexaploid syntenic shuttle mapping for the fruit-specific proanthocyanidin regulating gene (Astringency gene) in persimmon (Diopsyros kaki Thunb.)

柿子 (Diopsyros kaki Thunb.) 果实特异性原花青素调节基因（涩味基因）的二倍体/六倍体同线穿梭作图

• 发表时间: 2011
• 作者: T. Akagi;T. Tsujimoto;A. Sato;A. Kono;R. Tao;K. Yonemori
• 通讯作者: K. Yonemori

カキの遺伝学的解析におけるマメガキの有用性

日本牡蛎在牡蛎遗传分析中的用途

• 发表时间: 2010
• 作者: 武田萌;犬飼義明;狩野麻奈;山内章;辻本誠幸,赤木剛士,神崎真哉,米森敬三
• 通讯作者: 辻本誠幸,赤木剛士,神崎真哉,米森敬三

Practical marker-assisted selectiom using two SCAR markers for fruit astringency type in crosses of'Taiten'X PCNA cultivars in persimmon breeding

泰腾X PCNA品种杂交中两个SCAR标记对果实涩味类型的实用标记辅助选择在柿子育种中的应用

• 发表时间: 2013
• 期刊: Scientica Horticulturae
• 作者: Mitani N.;A. Kono;M. Yamada;A. Sato;S. Kobayashi;Y. Ban;T. Ueno;M. Shiraishi;S. Kanzaki;T. Tsujimoto;K. Yonemori
• 通讯作者: K. Yonemori

Fine genotyping of a highly polymorphic ASTRINGENCY-linked locus reveals variable hexasomic inheritance in persimmon (Diospyros kaki Thunb.) cultivars

• DOI: 10.1007/s11295-011-0432-0
• 发表时间: 2012-02-01
• 期刊: TREE GENETICS & GENOMES
• 影响因子: 2.4
• 作者: Akagi, Takashi;Tao, Ryutaro;Yonemori, Keizo
• 通讯作者: Yonemori, Keizo

Proanthocyanidin biosynthesis of persimmon (Diospyros kaki Thunb.) fruits

柿子 (Diospyros kaki Thunb.) 果实中原花青素的生物合成

• 发表时间: 2011
• 期刊: Scientia Horticulturae
• 影响因子: 4.3
• 作者: Akagi T.;A. Ikegami;K. Yonemori
• 通讯作者: K. Yonemori