Clarification of the mechanisms of regeneration of periodontal ligament using human clonal periodontal ligament progenitor cell line

利用人克隆性牙周膜祖细胞系阐明牙周膜再生机制

• 批准号: 19390486
• 负责人: MAEDA Hidefumi
• 金额: $ 12.4万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390486/
• 关键词: 歯内治療学; ヒト歯根膜細胞; 歯根膜幹細胞; angiotensin; ヒト歯根膜幹細胞株; 神経細胞; NGF; カルシウム; BMP2; 塩基性線維芽細胞増殖因子(bFGF); SaOS2

In this project, we have developed two clonal human periodontal ligament (PDL) stem/progenitor cell lines, and characterized them as cell elements responsible for PDL regeneration. Although both of cell lines expressed the same surface markers as bone marrow-derived mesenchymal stem cells, these cell lines specifically included Periostin and Scleraxis specific for PDL tissues. As scaffold elements, the materials containing calcium components effectively induced the differentiation of these two undifferentiated cell lines into cementoblastic/osteoblastic cells. Furthermore, as signaling elements, we clarified the efficacies of Basic Fibroblast Growth Factor and Transforming Growth Factor-β1 in PDL regeneration. And Angiotensin II that played important roles in regulating cardiovascular systems was suggested to affect tissue regeneration valuably. We also disclosed the functions of PDL stem/progenitor cells, indicating that our developed cell lines secreted Nerve Growth Factor to induce the neural differentiation, and neural migration, and inhibit apoptosis of neural cells. These results suggested that PDL tissue regeneration was accomplished by combining these three elements functionally.

在这个项目中，我们已经开发了两个克隆的人牙周膜（PDL）干/祖细胞系，并将其表征为负责PDL再生的细胞成分。虽然两种细胞系表达与骨髓源性间充质干细胞相同的表面标志物，但这些细胞系特别包括PDL组织特异性的骨膜蛋白和巩膜素。作为支架材料，含钙组分的材料有效地诱导这两种未分化细胞系分化为成牙骨质/成骨细胞。此外，我们阐明了碱性成纤维细胞生长因子和转化生长因子-β1作为信号元件在PDL再生中的功效。血管紧张素Ⅱ在心血管系统中起重要调节作用，对组织再生有重要影响。我们还揭示了PDL干/祖细胞的功能，表明我们开发的细胞系分泌神经生长因子，诱导神经分化和神经迁移，并抑制神经细胞的凋亡。这些结果表明，PDL组织再生是通过这三个元素的功能结合来完成的。

项目成果

What are the characteristics of PDL stem cells? The 5th International Symposium on "Dental and Craniofacial Morphogenesis and Tissue Regeneration: A View from Stem Cell Researches

PDL干细胞有何特点？

• 发表时间: 2010
• 作者: Maeda H;Fujii S;他7名
• 通讯作者: 他7名

歯根膜組織の発生とヒト歯根膜

牙周膜组织及人体牙周膜的发育

• 发表时间: 2007
• 作者: Anada T;Kamakura S;Shimauchi H;Suzuki O;et. al.;前田英史
• 通讯作者: 前田英史

歯根膜幹細胞はどんな特徴を持っているのだろうか?

牙周膜干细胞有什么特点？

• 发表时间: 2009
• 作者: 大山秀樹;小越菜保子;川辺陸記;安川陽子;安孫子宜光;前田英史
• 通讯作者: 前田英史

油症患者における歯周疾患ならびに口腔内色素沈着の疫学調査(第七報)

油病患者牙周病及口腔色素沉着的流行病学调查（第七次报告）

• 发表时间: 2009
• 期刊: 福岡医誌 100(5)
• 作者: 橋口勇;吉嶺嘉人;前田英史;後藤康治;藤井慎介;友清淳;吉田桐枝;西垣奏一郎;門野内聡;堀清美;奥村英彦;赤峰昭文
• 通讯作者: 赤峰昭文

医歯薬出版

石药出版社

• 发表时间: 2014
• 作者: 村上博和;齋藤貴之;笠松哲光
• 通讯作者: 笠松哲光