Analysis of jaw bone periosteum for prognosis of dental implant outcome

颌骨骨膜对牙种植结果预后的分析

• 批准号: 19390504
• 负责人: MATSUURA Takashi
• 金额: $ 11.73万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390504/
• 关键词: コラーゲン; 下顎骨; 皮質骨; 骨基質; コラーゲン架橋; リジン残基の水酸化; 上腕骨; 大腿骨; ミネラル; 骨粗鬆症; 水酸化リシン; マウス; 骨膜; 老化促進マウス; 骨代謝回転

Success of dental implant treatment at least depends on cortical bone matrix secreted by periosteal cells. To explore the aspect of cortical matrix of mandibular bone, this study performed biochemical analysis of collagen using two mouse models with different cortical width of mandibular bone, and compared cortical bone matrix of the mandible with that of the limbs in human cadavers. The mandibular cortical bone of senile osteoporotic mice (SAMP6), with comparison of their control mice (SAMR1), showed not only smaller cortical thickness but also less packed matrix histologically. Furthermore, SAMP6 exhibited lower content of collagen and higher extent of lysine hydroxylation of collagen, coincided with smaller diameter of collagen fibrils. As well, smaller amount of intermolecular cross-links of collagen was seen in SAMP6. These findings indicate that the mandibular cortical bone of SAMP6 with smaller bone volume of the cortex possesses less quantity and quality of the most abundant matrix protein, collagen. In human cadavers, the mandibular cortical bones showed higher contents of mineral and collagen but lower extent of lysine hydroxylation of collagen than the humeral and femoral cortical bones, suggesting better quality of bone matrix in the mandible compared with the limbs. In fact, values of the three indices varied among individuals. Thus, the cortical bone of the mandible exhibits inter-individual variation in matrix composition. The results of this study suggest that the analysis of cortical bone matrix, primarily collagen, which is the final product by periosteal cells, can disclose the various property of bone matrix and could be available for prognosis of dental implant outcome in future.

牙种植体治疗的成功至少取决于骨膜细胞分泌的皮质骨基质。为探讨下颌骨皮质基质的情况，本研究采用两种不同下颌骨皮质宽度的小鼠模型，对胶原进行了生化分析，并将下颌骨皮质基质与人尸体四肢皮质基质进行了比较。老年骨质疏松症小鼠（SAMP 6）的下颌骨皮质骨与其对照组小鼠（SAMR 1）相比，不仅表现出更小的皮质厚度，而且在组织学上也表现出更少的基质堆积。此外，SAMP6表现出较低的胶原含量和较高程度的胶原赖氨酸羟基化，与较小的胶原纤维直径相一致。同样，在SAMP6中观察到较少量的胶原分子间交联。这些结果表明，具有较小的皮质骨体积的SAMP 6的下颌骨皮质骨具有最丰富的基质蛋白，胶原蛋白的数量和质量较少。在人类尸体中，下颌骨皮质骨的矿物和胶原蛋白含量较高，但较低程度的赖氨酸羟基化的胶原比肱骨和股骨皮质骨，表明更好的骨基质的质量在下颌骨相比，四肢。事实上，这三个指数的值在个体之间是不同的。因此，下颌骨的皮质骨在基质组成上表现出个体间的差异。本研究结果提示，分析骨膜细胞最终产物皮质骨基质，主要是胶原，可以揭示骨基质的各种性质，并可用于未来牙种植体预后的预测。

项目成果

Lysine hydroxylation of collagen : an important posttranslational modification for physiological and pathophysiological conditions of general and oral connective tissues.

胶原蛋白的赖氨酸羟基化：一般和口腔结缔组织的生理和病理生理条件的重要翻译后修饰。

• 发表时间: 2007
• 期刊: Journal of Fukuoka Dental College 33
• 作者: Matsuura T;Katafuchi M;Tokutomi K;Sato H.
• 通讯作者: Sato H.

Quantity and quality of collagen are altered in mandibular bones in senile osteoporotic mice.

老年骨质疏松小鼠下颌骨中胶原蛋白的数量和质量发生改变。

• 发表时间: 2009
• 作者: Miyashita H;Mori S;Fukumoto Y;Sato A;Fukumoto M;Kawamura H;松浦尚志
• 通讯作者: 松浦尚志

Higher Contents of Mineral and Collagen but Lower of Hydroxylysine of Collagen in Mandibular Bone Compared with Those of Humeral and Femoral Bones in Human

与人体肱骨、股骨相比，下颌骨矿物质和胶原蛋白含量较高，但胶原蛋白羟赖氨酸含量较低

• DOI: 10.2485/jhtb.19.175
• 发表时间: 2010
• 期刊: Journal of Hard Tissue Biology
• 影响因子: 0.4
• 作者: Michiko Sasaki;T. Matsuura;Michitsuna Katafuchi;Kentaro Tokutomi;Hironobu Sato
• 通讯作者: Hironobu Sato

老年性骨粗鬆症モデルマウスの下顎骨のコラーゲン線維形態と分子間架橋

老年骨质疏松模型小鼠下颌骨胶原纤维形态及分子间交联

• 发表时间: 2008
• 作者: 松浦尚志;他
• 通讯作者: 他

Podocan affects cell proliferation and in vitro mineralization possibly by inducing cellular senescence in MC3T3-EI cell

Podocan 可能通过诱导 MC3T3-EI 细胞衰老来影响细胞增殖和体外矿化

• 发表时间: 2007
• 作者: Katafuchi M;et. al.
• 通讯作者: et. al.