The expression and function of double-stranded RNA-dependent protein kinase (PKR) in rat intestinal epithelial cells

双链RNA依赖性蛋白激酶(PKR)在大鼠肠上皮细胞中的表达及功能

• 批准号: 19590201
• 负责人: DOI Yoshiaki
• 金额: $ 2.75万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19590201/
• 关键词: 細胞分化; 小腸上皮細胞; アポトーシス; 二本鎖RNA依存性プロテインキナーゼ(PKR); ハイドロコルチゾン; STAT-1; PKR; ラット小腸上皮細胞; PKR阻害薬; IEC6細胞; 免疫組織化学; ヒドロコルチゾン; 合成二本鎖RNA; 細胞培養; ラット壁細胞; real time RT-PCR; ウェスタンブロッティング; 分化誘導; 走査型電子顕微鏡

Intestinal epithelial cells (IECs) are exposed to microbial and viral products, and serve as essential barriers to them. Since double-stranded RNA-dependent protein kinase (PKR) is involved in cellular antiviral response, cell differentiation and apoptosis, we tried to investigate the expression and roles of PKR in rat IECs. In this study, we showed that the expression of PKR in IECs of adult rats. We also demonstrated that PKR was expressed in cultured rat IECs. The level of PKR protein expression and the activity of alkaline phosphatase (ALP) increased in the cultured IECs in a time-dependent manner. Treatment with PKR inhibitor decreased ALP activity in the IECs. The addition of synthetic double-stranded RNA induced apoptosis in a dose-dependent manner in these cells. Treatment with hydrocortisone also provoked suppression of PKR expression in such cells. Thus, we concluded that PKR is expressed in IECs as potent barriers to antigens and is a possible modulator of the differentiation and apoptosis in rat IECs.

肠道上皮细胞(IECs)暴露在微生物和病毒产品中，是它们的基本屏障。鉴于双链RNA依赖蛋白激酶(PKR)参与细胞抗病毒反应、细胞分化和细胞凋亡，我们试图研究PKR在大鼠IECS中的表达及其作用。在本研究中，我们发现了PKR在成年大鼠IECs中的表达。我们还证明在培养的大鼠IECs中有PKR的表达。培养的IECs中PKR蛋白表达水平和碱性磷酸酶(ALP)活性呈时间依赖性增加。用PKR抑制剂处理后，IECS内ALP活性降低。加入人工合成的双链RNA以剂量依赖的方式诱导这些细胞的凋亡。氢化可的松也抑制了这些细胞中PKR的表达。因此，我们认为PKR在IECS中的表达是对抗原的有效屏障，并且可能是大鼠IECS分化和凋亡的调节因子。

项目成果

An integrin-activating peptide, PHSRN, ameliorates inhibitory effects of conventional peritoneal dialysis fluids on peritoneal wound healing

整合素激活肽 PHSRN 可改善传统腹膜透析液对腹膜伤口愈合的抑制作用

• 发表时间: 2010
• 期刊: Nephrol. Dial. Transplant. 25
• 作者: Miyamoto;T;et al.
• 通讯作者: et al.

胎盤栄養から母乳栄養への移行期における小腸吸収上皮細胞の形態と機能の変化

胎盘营养向母乳营养过渡期间小肠吸收上皮细胞形态和功能的变化

• 发表时间: 2008
• 作者: 馬場良子;他
• 通讯作者: 他

Differential expression of protein phosphatase type 1 isotypes and nucleolin during cell-cycle arrest

细胞周期停滞期间蛋白磷酸酶 1 型同种型和核仁素的差异表达

• 期刊: Cell Biochemistry and Function (in press)
• 作者: Nakamura;T.;Arai;Y.;Umehara;H.;Masuhara;M.;Kimura;T.;Taniguchi;H.;Sekimoto;T.;Ikawa;M.;Yoneda;Y.;Okabe;M.;Tanaka;S.;Shiota;K.;Nakano;T.;羽毛田慈之;Ozaki A;Hakeda Y.;Ozaki A;Ozaki A;Haneji T;Okamura H;Kito S;Morimoto H;Yoshida K;Okamura H;Amorim BR;Morimoto H
• 通讯作者: Morimoto H

Expressions of the multidrug resistance-related proteins in the rat olfactory epithelium: A possible role in the phaseIIIxenobiotic metabolizing function

大鼠嗅上皮中多药耐药相关蛋白的表达：在III期异生物质代谢功能中的可能作用

• 发表时间: 2010
• 期刊: Neurosci. Lett. Vol.468、No.2
• 作者: Kudo;H. 、Doi;Y. 、Fujimoto;S.
• 通讯作者: S.

Expressions of the multidrug resistance-related proteins in the rat olfactory epithelium : A possible role in the phase III xenobiotic metabolizing function

大鼠嗅上皮中多药耐药相关蛋白的表达：在 III 期外源性代谢功能中的可能作用

• 发表时间: 2010
• 期刊: Neurosci. Lett. 468
• 作者: Kudo;H;et al.
• 通讯作者: et al.