Application of Monolithic Porous Layer on SiWafer -Toward Cell Manipulation for Biotechnology-

在硅芯片上制作了多孔芯片阵列，并通过真空抽吸在多孔芯片上展示了细胞阵列。可以预料，多孔区域很容易被培养基中含有的一些物质堵塞。然后测量通过多孔区域的压力降，并且发现使用新鲜过滤的培养基可以获得足够的抽吸流体体积。证明了细胞固定化，并且发现在固定化后通过施加强吸力可以诱导细胞破碎。

Through-chip porous area array was made on a Si chip and cellular array was demonstrated by vacuum suction on the through-chip porous areas. It was expected that the porous area was easily blocked by some substances contained in a cultivation medium. Then the pressure drop through the porous area was measured and it was found that enough suctional fluidic volume can be obtained with a fresh filtrated cultivation medium. Cell immobilization was demonstrated and it was found that cell fragmentation could be induced after the immobilization by applying strong suction.