Application of Monolithic Porous Layer on SiWafer -Toward Cell Manipulation for Biotechnology-

在硅片上制作了穿透芯片多孔区阵列，并通过对穿透芯片多孔区的真空抽吸演示了蜂窝状阵列。预计多孔区域很容易被培养介质中的某些物质堵塞。然后测量了通过多孔区的压力降，发现用新鲜过滤的培养介质可以获得足够的吸液量。对细胞进行了固定化实验，发现在施加强吸力的情况下，固定化后的细胞会发生碎裂。

Through-chip porous area array was made on a Si chip and cellular array was demonstrated by vacuum suction on the through-chip porous areas. It was expected that the porous area was easily blocked by some substances contained in a cultivation medium. Then the pressure drop through the porous area was measured and it was found that enough suctional fluidic volume can be obtained with a fresh filtrated cultivation medium. Cell immobilization was demonstrated and it was found that cell fragmentation could be induced after the immobilization by applying strong suction.