Functional analyses of the bHLH transcription factor DEC2 in cardiac and neural differentiations.

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We studied about the molecular mechanisms by which Dec2 regulates gene expression. Gel retardation and luciferase assays showed that Dec2 preferentially binds to class B E-box elements (CACGTG) as a homodimer and represses the transcription of target genes in a histone deacetylase (HDAC)-dependent manner. In contrast, Dec2 repressed a MyoD-activated promoter activity of muscle creatine kinase gene through class A E-box in an HDAC1-independent manner. These findings indicate that Dec2 employs multiple mechanisms -including DNA-binding and protein-protein interactions-to achieve E-box-dependent transcriptional repressions.