Die Rolle transkriptioneller Regulation beim Start des Zellzyklus der Hefe

转录调控在酵母细胞周期启动中的作用

• 批准号: 5369295
• 负责人: Professor Dr. Christian Koch
• 金额: --
• 依托单位: Lehrstuhl für Biochemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 1999
• 资助国家: 德国
• 起止时间: 1998-12-31 至 2001-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5369295?language=en
• 关键词: Die; Rolle; transkriptioneller; Regulation; beim

Entry into cell cycle in late G1 is a central regulatory step of the eukaryotic cell cycle. In yeast, the timing of S-phase entry is largely determined by transcriptional activation of G1 cyclins und DNA synthesis genes as cells reach a critical size. Their expression is regulated by the cyclin dependent kinase Cdc28p and depends on two transcription factor complexes called SBF (Swi4/Swi6) and MBF (Mbp1/Swi6). In the proposed project we study the function and regulation of these transcription factors. We have genetically and biochemically identified novel proteins (Ctr9p and Paf1p) required for gene expression in late G1. Their phyiological role is being analysed. The aims of the project are to elucidate how transcriptional activation in late G1 is coordinated with cell size and cellular growth.

G1晚期进入细胞周期是真核细胞周期的中心调控步骤。在酵母中，当细胞达到临界大小时，s期进入的时间在很大程度上取决于G1周期蛋白和DNA合成基因的转录激活。它们的表达受细胞周期蛋白依赖性激酶Cdc28p调控，并依赖于两种转录因子复合物SBF （Swi4/Swi6）和MBF （Mbp1/Swi6）。在本项目中，我们将研究这些转录因子的功能和调控。我们已经通过遗传和生化鉴定出G1晚期基因表达所需的新蛋白（Ctr9p和Paf1p）。他们的生理作用正在被分析。该项目的目的是阐明G1晚期的转录激活如何与细胞大小和细胞生长协调。