Analysis of the siderophore genes in Nocardia

点击翻译按钮获取中文摘要

Recently, we have determined the complete genome sequence of Nocardia farcinica IFM 10152 and found gene clusters (nbt gene clusters) thought to be responsible for the biosynthesis of siderophore in the genome. In this study, as expected, a siderophore was isolated from this strain and identified as nocobactin NA. The chemical structure is in good agreement with the gene organization of the nbt gene clusters. Furthermore, gene disruption and heterologous expression experiments demonstrated that nbt gene clusters were responsible for the biosynthesis of nocobactin NA.To our knowledge, this is the first example of the biosynthetic genes and pathway of mycobactin type siderophore in the Nocardia. Furthermore, this work highlights the importance of genome analysis in combination with metabolite analysis.