Development of a new mass fragmentation analysis for understanding the diversity of metabolite

开发新的质量碎片分析以了解代谢物的多样性

• 批准号: 20200062
• 负责人: SUZUKI Hideyuki
• 金额: $ 20.38万
• 依托单位: Kazusa DNA Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20200062/
• 关键词: 代謝物多様性; 酸化反応; メタボロミクス; 生物種間比較; 進化; 網羅的解析

In order to understand the diversity of metabolites produced by organisms, we have investigated three topical studies focused on oxidative reaction of metabolite. 1) We attempted to isolate a cDNA of flavonoid 8-hydroxylase from Lotus japonicus. This is the first identification of a flavonoid 8-hydroxylase cDNA from any plant species, and also this enzyme is a novel type of flavonoid hydroxylase. 2) We established a system to analyze metabolites found in Lotus japonicus that grows under oxygen-18 isotope and light conditions (16-h light/8-h dark cycles) at 22℃ for four weeks. 3) We developed a comprehensive metabolite network system based on MS/MS fragmentation analysis to classify the structural group of metabolites.

为了了解生物体产生的代谢产物的多样性，我们调查了三个主题研究集中在代谢产物的氧化反应。1)我们试图从百脉根中分离黄酮8-羟化酶的cDNA。这是第一次从植物中鉴定出类黄酮8-羟化酶cDNA，也是一种新型的类黄酮羟化酶。2)我们建立了一个系统来分析在氧-18同位素和光照条件下（16小时光照/8小时黑暗循环）在22℃下生长四周的百脉根中发现的代谢物。3)我们开发了一个全面的代谢物网络系统的基础上MS/MS裂解分析的代谢物的结构组进行分类。

项目成果

公開前につき非開示

发布前未披露

• 发表时间: 2010