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Development of a new mass fragmentation analysis for understanding the diversity of metabolite

Development of a new mass fragmentation analysis for understanding the diversity of metabolite
开发新的质量碎片分析以了解代谢物的多样性
批准号：
20200062
负责人：
SUZUKI Hideyuki
金额：
$20.38万
依托单位：
Kazusa DNA Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
财政年份：
2008
资助国家：
日本
项目状态：
已结题
起止时间：
2008 至 2010
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20200062/
关键词：
代謝物多様性酸化反応メタボロミクス生物種間比較進化網羅的解析

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中文摘要
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英文摘要
In order to understand the diversity of metabolites produced by organisms, we have investigated three topical studies focused on oxidative reaction of metabolite. 1) We attempted to isolate a cDNA of flavonoid 8-hydroxylase from Lotus japonicus. This is the first identification of a flavonoid 8-hydroxylase cDNA from any plant species, and also this enzyme is a novel type of flavonoid hydroxylase. 2) We established a system to analyze metabolites found in Lotus japonicus that grows under oxygen-18 isotope and light conditions (16-h light/8-h dark cycles) at 22℃ for four weeks. 3) We developed a comprehensive metabolite network system based on MS/MS fragmentation analysis to classify the structural group of metabolites.
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发表时间： 2010
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作者： []
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