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Development of a new mass fragmentation analysis for understanding the diversity of metabolite

Development of a new mass fragmentation analysis for understanding the diversity of metabolite
开发新的质量碎片分析以了解代谢物的多样性
批准号:
20200062
负责人:
SUZUKI Hideyuki
金额:
$20.38万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010

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项目成果

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中文摘要
翻译
为了了解生物体产生的代谢物的多样性,我们调查了三个专题研究,重点是代谢物的氧化反应。1)我们尝试从莲花中分离出一个黄酮类8-羟基酶的基因。这是首次从植物中鉴定出一个黄酮类8-羟基酶基因,而且该酶是一种新型的类黄酮羟基酶。2)我们建立了一套系统来分析在22℃的氧-18同位素和光照条件(16小时光/8小时暗周期)下生长四周的莲花中的代谢物。3)建立了基于MS/MS裂解分析的代谢物网络系统,对代谢物的结构群进行了分类。
英文摘要
In order to understand the diversity of metabolites produced by organisms, we have investigated three topical studies focused on oxidative reaction of metabolite. 1) We attempted to isolate a cDNA of flavonoid 8-hydroxylase from Lotus japonicus. This is the first identification of a flavonoid 8-hydroxylase cDNA from any plant species, and also this enzyme is a novel type of flavonoid hydroxylase. 2) We established a system to analyze metabolites found in Lotus japonicus that grows under oxygen-18 isotope and light conditions (16-h light/8-h dark cycles) at 22℃ for four weeks. 3) We developed a comprehensive metabolite network system based on MS/MS fragmentation analysis to classify the structural group of metabolites.
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发表时间: 2010
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作者: []
通讯作者:
Studies on catabolic pathway of spermidine in E. coli
  • 批准号:
    15K07357
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $3.16万
  • 财政年份:
    2015
  • 负责人:
    SUZUKI Hideyuki
  • 依托单位:
Development of training method to enhance problem-oriented discussion skills through integration of sympathetic and logical aspect of thinking
  • 批准号:
    23300295
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $11.4万
  • 财政年份:
    2011
  • 负责人:
    SUZUKI Hideyuki
  • 依托单位:
Functional analysis of isoflavonoid biosynthesis related transcription factors using gene co-expression network analysis
  • 批准号:
    23510272
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $3.49万
  • 财政年份:
    2011
  • 负责人:
    SUZUKI Hideyuki
  • 依托单位:
Development of tool and method for enhancing note-taking skills for collaborative learning
  • 批准号:
    23650525
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.33万
  • 财政年份:
    2011
  • 负责人:
    SUZUKI Hideyuki
  • 依托单位:
海外基金