Studies on the molecular mechanisms for Marek's disease virus to increase its virulence in the field

马立克氏病病毒野外毒力增强的分子机制研究

• 批准号: 20380157
• 负责人: OHASHI Kazuhiko
• 金额: $ 12.06万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20380157/
• 关键词: 病原微生物; マレック病; マレック病ウイルス

To clarify the molecular mechanisms for Marek's disease virus (MDV) to increase its virulence, several viral genes of MDV strains recently isolated from the field were analyzed. Diversities/polymorphisms were identified in one of the MDV genes, meq, that could be potentially related to the increase in MDV virulence. When we analyzed the effects of these diversities/polymorphisms in the meq gene on the functions of the meq gene product (Meq), the amino acid substitutions resulted from the diversities/polymorphisms significantly altered the transactivation and transforming activities of Meq. These findings suggest that the diversities/polymorphisms in the meq gene could contribute to the recent increase in MDV virulence in the field.

为了阐明马立克氏病病毒（MDV）增强毒力的分子机制，对近年来从马立克氏病病毒（MDV）分离的几个毒株进行了基因分析。在MDV的一个基因meq中发现了差异/多态性，这可能与MDV毒力的增加有关。当我们分析meq基因中的这些二聚体/多态性对meq基因产物（Meq）的功能的影响时，由二聚体/多态性导致的氨基酸取代显著改变了Meq的反式激活和转化活性。这些结果表明，meq基因的双链/多态性可能有助于最近在该领域的MDV毒力的增加。

项目成果

マレック病ウイルスmeq遺伝子の多型に伴うMeqタンパクの転写活性化能の変化

马立克氏病病毒meq基因多态性导致Meq蛋白转录激活能力的变化

• 发表时间: 2008
• 作者: 橋口知幸;村田史郎;岡田宰;加納里佳;今内覚;小沼操;大橋和彦
• 通讯作者: 大橋和彦

Microarray Analysis of Host Immune Responses to Marek's Disease Virus Infection in Vaccinated Chickens

• DOI: 10.1292/jvms.71.603
• 发表时间: 2009-05-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Kano, Rika;Konnai, Satoru;Ohashi, Kazuhiko
• 通讯作者: Ohashi, Kazuhiko

Prevalence of fowl glioma-inducing virus in chickens of zoological gardens in Japan and nucleotide variation in the env gene

• DOI: 10.1292/jvms.70.469
• 发表时间: 2008-05-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Hatai, Hitoshi;Ochiai, Kenji;Umemura, Takashi
• 通讯作者: Umemura, Takashi

Identification and characterization of antimicrobial peptides, defensin, in the taiga tick Ixodes persulcatus

针叶林蜱 Ixodes persulcatus 中抗菌肽、防御素的鉴定和表征

• 发表时间: 2009
• 期刊: Insect Mol.Biol. 18
• 作者: Saito Y.;Konnai S.;Yamada S.;Imamura S.;Nishikado H.;Ito T.;Onuma M.;Ohashi K.
• 通讯作者: Ohashi K.

Regulation of the interferon gamma expression by an onocoprotein of Marek's disease virus in wild waterfowls and chickens

马立克氏病病毒的癌蛋白对野生水禽和鸡中干扰素γ表达的调节

• 发表时间: 2010
• 作者: 松原綾子;村田史郎;加納里佳;橋口知幸;伊勢崎政美;河東あゆ美;小沼操;今内覚;大橋和彦
• 通讯作者: 大橋和彦