Study on Induction of Th2 response and allergic inflammation by basophils

嗜碱性粒细胞诱导Th2反应和过敏性炎症的研究

• 批准号: 20390145
• 负责人: NAKANISHI Kenji
• 金额: $ 12.73万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390145/
• 关键词: 好塩基球; Th2細胞; 抗原提示; アレルギー性炎症; アレルゲン; IgE複合体; 抗原提示細胞; Th2/IgE応答; アレルゲン/IgE複合体; 寄生虫感染; allergen-IgE複合体; Th2細胞の誘導; MHC class II; CD80; 86

Basophils, accounting for less than 1% of total circulating granulocytes, constitutively express FcεR1, and upon crosslinking of FcεR1-bound IgE with multivalent antigens, immediately release various kinds of effector molecules such as histamine and lipid mediators, and Th2-associated cytokines such as IL-4 and IL-13, causing immediate type hypersensitivity. Furthemore, basophils express IL-18R and IL-33R and very strongly produce IL-4 and IL-13 in response to IL-18 and/or IL-33. We have demonstrated that basophils express MHC class II and CD80/86, have the potential to take-up and process protein antigen (Ag), particularly Ag-IgE complexes, and to present peptide with MHC class II and produce IL-4, revealing that basophils are APC, which selectively induce Th2 cells.

嗜碱性粒细胞仅占循环粒细胞总数的不到1%，其组成性表达FcεR1，当FcεR1结合的Ig E与多价抗原交联后，立即释放各种效应分子如组胺和脂质介质，以及Th2相关细胞因子如IL-4和IL-13，引起即刻型超敏反应。此外，嗜碱性粒细胞表达IL-18R和IL-33R，并强烈地产生IL-4和IL-13，以响应IL-18和/或IL-33。我们已经证明嗜碱性粒细胞表达MHC-II类和CD80/86，具有摄取和处理蛋白质抗原(Ag)，特别是Ag-IgE复合体的能力，并与MHC-II类呈递多肽并产生IL-4，揭示嗜碱性粒细胞是APC，选择性地诱导Th2细胞。

项目成果

In vivo administration of IL-33 induces goblet cells capable of expelling Nippostrongylus brasiliensis in the absence of adaptive immune system.

体内给予IL-33可诱导杯状细胞在缺乏适应性免疫系统的情况下能够排出巴西圆线虫。

• 发表时间: 2008
• 作者: Yasuda;K.;Sasaki;Y.;Kondo;Y.;Matsumoto;M.;Yoshimoto;T. Nakanishi;K.
• 通讯作者: K.

Basophils play a pivotal role in the expulsion of gastrointestinal nematode Strongyloides venezuelensis.

嗜碱性粒细胞在驱除胃肠道线虫委内瑞拉类圆线虫方面发挥着关键作用。

• 发表时间: 2010
• 作者: Matsumoto M;Yasuda K;Kubo M;Yoshimoto T;Nakanishi K.
• 通讯作者: Nakanishi K.

Basophils are potentantigen-presenting cells which selectively induce Th2 cells.

嗜碱性粒细胞是有效的抗原呈递细胞，可选择性诱导 Th2 细胞。

• 发表时间: 2010
• 作者: 武田裕;中島和江;中西憲司
• 通讯作者: 中西憲司

Administration of IL-33 induces airway hyperresponsiveness and goblet cell hyperplasia in the lungs in the absence of adoptive immune system.

在缺乏过继性免疫系统的情况下，施用 IL-33 会​​诱导气道高反应性和肺部杯状细胞增生。

• 发表时间: 2009
• 作者: Yasuda K;Kondo Y;Yoshimoto T;Fujimoto J;Nakanishi K
• 通讯作者: Nakanishi K

Basophils contribute to TH2-IgE responses in vivo via IL-4 production and presentation of peptide-MHC class II complexes to CD4+ T cells

• DOI: 10.1038/ni.1737
• 发表时间: 2009-07-01
• 期刊: NATURE IMMUNOLOGY
• 影响因子: 30.5
• 作者: Yoshimoto, Tomohiro;Yasuda, Koubun;Nakanishi, Kenji
• 通讯作者: Nakanishi, Kenji