Induction of intermediate mesoderm and kidney progenitors from embryonic stem cells

本项目旨在从胚胎干细胞中诱导含有肾元祖细胞的中胚层和后胚层间质。我们产生了一个敲入小鼠品系，其中这两个肾脏前体种群被GFP标记。筛选后的gfp阳性细胞在Wnt4刺激下进行集落形成实验。我们发现胚胎在E9。5英镑，但不是8英镑。5、含有集落形成细胞。由于由胚胎干细胞诱导的gfp阳性细胞不能形成集落，我们假设它们可能对应于E8。5中间中胚层；需要进一步研究从胚胎干细胞中获得肾元祖细胞。

This project aims at induction of the intermediate mesoderm and metanephric mesenchyme, which contains nephron progenitors, from embryonic stem(ES) cells. We generated a knock-in mouse strain in which these two kidney precursor populations were marked with GFP. Sorted GFP-positive cells were subjected to the colony-forming assay upon Wnt4 stimulation. We revealed that embryos at E9. 5, but not at E8. 5, contained colony-forming cells. As GFP-positive cells induced from ES cells were unable to form colonies, we assume that they may correspond to the E8. 5 intermediate mesoderm. Further investigation is needed to derive nephron progenitors from ES cells.