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Development of High-Performance Two-Dimensional Electrophoresis Using Low-Concentration Gel and Its Application

Development of High-Performance Two-Dimensional Electrophoresis Using Low-Concentration Gel and Its Application
低浓度凝胶高性能二维电泳的研制及其应用
批准号:
20550072
负责人:
ODAKE Tamao
金额:
$3.16万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010

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中文摘要
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英文摘要
The researchers have developed a new isoelectric focusing(IEF) gel supported by a multifilament as a first-dimensional IEF gel of two-dimensional electrophoresis(2-DE), which can separate proteins with high-resolution. The multifilament-supporting(MFS) gel could overcome difficulty in handling the IEF gel, prepare low-concentration gel to separate high-molecular-weight proteins. In this study, to improve operability and reproducibility, support of the low-concentration gel, method of polymerization, and introduction of immobilized pH gradient were investigated. The 2-DE system using the low-concentration MFS gel was proved to be useful for proteome analysis.
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DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [富谷頼行, 高橋透, 壹岐伸彦, 星野仁, 小竹玉緒,宮田梢,植木悠二,堀田弘樹,片貝秋雄,玉田正男,角田欣一]
通讯作者: 小竹玉緒,宮田梢,植木悠二,堀田弘樹,片貝秋雄,玉田正男,角田欣一
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DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [Y.Inaba, T.Kawakami, S.Aimoto, T.Ikegami, T.Takeuchi, T.Nakazawa, S.Yano, Y.Mikata, 鈴木孝義, 亀田恭男,天羽優子,臼杵毅, 清水祐毅,浅野淳也,佐伯俊彦]
通讯作者: 清水祐毅,浅野淳也,佐伯俊彦
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DOI: --
发表时间: 2008
期刊:
影响因子: --
作者: [S.Yamazaki, S.Morikawa, K.Miyazaki, M.Takebayashi, Y.Yamamoto, T.Morimoto, K.Kakiuchi, Y.Mikata, 富谷頼行・高橋透・壹岐伸彦・星野仁, Ryoji Mitsuhashi, N. Iki ; K. Kano ; H. Hoshino, 小竹玉緒,植木悠二,片貝秋雄,玉田正男,宮田梢,櫻井佳祐,堀田弘樹,角田欣一]
通讯作者: 小竹玉緒,植木悠二,片貝秋雄,玉田正男,宮田梢,櫻井佳祐,堀田弘樹,角田欣一
DOI: 10.1007/s10616-008-9122-7
发表时间: 2008-06
期刊: CYTOTECHNOLOGY
影响因子: 2.2
作者: [Saheki, Toshihiko, Ito, Hitomi, Sekiguchi, Akihiro, Nishina, Atsuyoshi, Sugiyama, Toshihiro, Izumi, Takashi, Kojima, Itaru]
通讯作者: Kojima, Itaru
17
    Development of High-Performance Two-Dimensional Gel Electrophoresis Using Low-Concentration Polyacrylamide Gel Supported by Multifilament Yarns
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