Screening of thermophilic aldolases and application for the synthesis of sugar related materials
Screening of thermophilic aldolases and application for the synthesis of sugar related materials
批准号:
20560730
负责人:
SAKURABA Haruhiko
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010
中文摘要
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英文摘要
The hyperthermophilic 2-deoxy-D-ribose-5-phosphate aldolase (DERA) gene was amplified from environmental DNA by PCR. These fragments were swapped with corresponding sequence of the known hyperthermophilic DERA gene and the expression vector for the chimera enzyme was constructed. The specific activity of the recombinant chimera DERA produced in Escherichia coli was two times higher than that of the wild type enzyme. In addition, we succeeded in determination of the crystal structure of hyperthermophilic 2-keto-3-deoxygluconate aldolases.
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Crystallization and preliminary X-ray analysis of a novel dye-linked L-proline dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix
来自需氧超嗜热古菌 Aeropyrum pernix 的新型染料连接 L-脯氨酸脱氢酶的结晶和初步 X 射线分析
DOI:
--
发表时间:
2010
期刊:
Acta.Cryst.F Vol.66
影响因子:
--
作者:
[T.Satomura, H.Sakuraba, Y.Hara, T.Ohshima]
通讯作者:
T.Ohshima
Structure of L-aspartate oxidase from the hyperthermophilic archaeon Sulfolobus tokodaii
超嗜热古菌 Sulfolobus tokodaii 的 L-天冬氨酸氧化酶的结构
DOI:
--
发表时间:
2008
期刊:
Biochim Biophys Acta 1784
影响因子:
--
作者:
[Sakuraba, H., Yoneda, K., Asai, I., Tsuge, H., Katunuma, N., Ohshima, T]
通讯作者:
T
DOI:
10.1111/j.1742-4658.2010.07916.x
发表时间:
2010-12
期刊:
The FEBS Journal
影响因子:
--
作者:
[K. Yoneda;H. Sakuraba;Ikuo Muraoka;T. Oikawa;T. Ohshima]
通讯作者:
K. Yoneda;H. Sakuraba;Ikuo Muraoka;T. Oikawa;T. Ohshima
DOI:
10.1007/s00253-010-2914-7
发表时间:
2011-02
期刊:
Applied Microbiology and Biotechnology
影响因子:
5
作者:
[T. Satomura;Xiaodong Zhang;Y. Hara;Katsumi Doi;H. Sakuraba;T. Ohshima]
通讯作者:
T. Satomura;Xiaodong Zhang;Y. Hara;Katsumi Doi;H. Sakuraba;T. Ohshima
The first crystal structure of L-lysine 6-dehydrogenase as an NAD-dependent amine dehydrogenase
L-赖氨酸 6-脱氢酶作为 NAD 依赖性胺脱氢酶的第一个晶体结构
DOI:
--
发表时间:
2010
期刊:
J.Biol.Chem. Vol.285
影响因子:
--
作者:
[K.Yoneda, J.Fukuda, H.Sakuraba, T.Ohshima]
通讯作者:
T.Ohshima
共 14 条
Application of Hyperthennophilir 2-Deoxy-D-Riltose-5-Phosphate Aldolase
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批准号:17613002
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.43万
-
财政年份:2005
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负责人:SAKURABA Haruhiko
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依托单位:
Studies on hyperthermostable aldolase : characteristics, structure and application
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批准号:15560677
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.37万
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财政年份:2003
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负责人:SAKURABA Haruhiko
-
依托单位:
Studies on NAD synthetic pathway of hyperthermophile based on genome information
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批准号:13680716
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项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.3万
-
财政年份:2001
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负责人:SAKURABA Haruhiko
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依托单位:
海外基金