Study on the structure and function of glucosidases acting on glycochains

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The purpose of this research was to elucidate the structure-function relationship of alpha-glucosidases and related enzymes, which act on the N-linked oligosaccharides. This research also investigated the relationship between the N-linked oligosaccharides and the protein structure. The three-dimensional structure of an enzyme homologous to processing alpha-glucosidase I has been determined, and the enzyme was shown to be useful for producing oligosaccharides. The expression system of a fungal processing alpha-glucosidase I was constructed, and the enzymatic properties were determined. We also investigated some enzymatic properties of a bacterial enzyme homologous to endo-alpha-1,2-mannosidase, an enzyme potentially physiologically related to processing alpha-glucosidases. This was the first report on the enzymatic characterization of a bacterial endo-alpha-1,2-mannosidase.