Single-molecule tracking study of the mechanisms of signal transduction in lipid rafts of cell plasma membranes

细胞质膜脂筏信号转导机制的单分子追踪研究

• 批准号: 20570154
• 负责人: SUZUKI KEenichi
• 金额: $ 3.16万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570154/
• 关键词: 1分子観察; 細胞膜; ラフト; シグナル伝達; 受容体

It has turned out that in steady-state cell plasma membranes, GPI-anchored proteins formed transient homo-dimers based on the extracellular protein-protein interactions. In the presence of protein-protein interactions, lipid interactions were found to facilitate the formation of the homo-dimers, while this was not observed in the absence of the protein interactions. Furthermore, it was found that upon ligation of GPI-anchored receptors, very stable homo-oligomers were formed, the oligomers recruited other raft lipid, ganglioside, and induced raft-like structure in the oligomers. The formation of the raft-like structure in the oligomers was essential for the downstream signaling such as intracellular calcium response.

事实证明，在稳态细胞质膜中，GPI 锚定蛋白基于细胞外蛋白-蛋白相互作用形成瞬时同源二聚体。在存在蛋白质-蛋白质相互作用的情况下，发现脂质相互作用促进同源二聚体的形成，而在不存在蛋白质相互作用的情况下没有观察到这一点。此外，发现在连接GPI锚定受体后，形成非常稳定的同源寡聚物，该寡聚物募集了其他筏脂质、神经节苷脂，并在寡聚物中诱导了筏状结构。寡聚物中筏状结构的形成对于下游信号传导（例如细胞内钙反应）至关重要。

项目成果

Mechanism for signal transduction in the induced-raft domains as revealed by single molecule tracking

单分子追踪揭示诱导筏域信号转导机制

• 发表时间: 2008
• 期刊: Trends in Glycoscience and Glycotechnology 20(116)
• 作者: K. G. N. Suzuki;and A. Kusumi
• 通讯作者: and A. Kusumi

How does the meso-scale raft domain exist and transform to work? : single-molecule imaging study

中观尺度筏域如何存在并转变以发挥作用？

• 发表时间: 2010
• 作者: K. G. N. Suzuki;A. Kusumi;鈴木健一;鈴木健一;鈴木健一
• 通讯作者: 鈴木健一

Full characterization of GPCR monomer-dimer dynamic equilibrium by single molecule imaging.

• DOI: 10.1083/jcb.201009128
• 发表时间: 2011-02-07
• 期刊: The Journal of cell biology
• 作者: Kasai RS;Suzuki KG;Prossnitz ER;Koyama-Honda I;Nakada C;Fujiwara TK;Kusumi A
• 通讯作者: Kusumi A

1分子観察で初めて見えてくる細胞膜ラフト構造とシグナル伝達機構

首次通过单分子观察揭示细胞膜筏结构和信号转导机制

• 发表时间: 2011
• 作者: K. G. N. Suzuki;A. Kusumi;鈴木健一
• 通讯作者: 鈴木健一

Lipid-stabilized homo-dimers of GPI-anchored proteins based on ectodomain protein interactions

基于胞外域蛋白相互作用的 GPI 锚定蛋白的脂质稳定同二聚体

• 发表时间: 2010
• 作者: 鈴木健一;他;鈴木健一;鈴木健一
• 通讯作者: 鈴木健一