Development of new molecular technique for determination of death by drowning

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We determined some bacterioplankton as markers for diagnosis of drowning and tried to develop a new method using TaqMan-PCR : the genus Aeromomas (A.hydrophila, A.salmonicida), Vibrio (V.fischeri, V.harveyi, V.parahaemolyticus), Photobacterium (P.damselae, P.phosphoreum, P.leiognathi). We adopted the method of Triplex-PCR, which can detect three type of bacterioplankton simultaneously, and PCR was performed by 40 cycles using 7500 Real Time PCR System. Present method was specific for standard strains of above bacterioplankton. Moreover, bacterioplankton was detectable in blood or tissue from cadavers retrieved from the sea (n=5), rivers (fresh water, n=8),near estuaries (n=2) by the new method.