畜産食品タンパク質をモデルとした合成ペプチドによる生体調節機能の解明

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Oxidative stress has been implicated in the mucosal inflammation of the gastrointestinal tract. Several studies have described the antioxidant activity of proteins from several animal and plant sources. The antioxidant activity of peptides generated from the hydrolysis of various proteins, such as egg white protein, egg yolk protein, soy protein, and milk casein has been reported. Recent studies have described the antioxidant activity of egg yolk protein phosvitin and phosvitin phosphopeptides (PPP). An inflammatory chemokine, interleukin-8 (IL-8), is induced by oxidative stress, and the antioxidants have been shown to inhibit IL-8 expression. Hydrogen peroxide is known to increase IL-8 production in human epithelial cell lines. We investigated the effect a chemically synthesized poly-phosphoserine as a moldel of PPP on the production of IL-8 induced by hydrogen peroxide in intestinal epithelial Caco-2 cells. The chemokine production was measured by enzyme-linked immunosorbent assay (ELISA). We found that the synthesized poly-phosphoserine significantly inhibited the hydrogen peroxide-induced IL-8 production in Caco-2 cells. Moreover, the inhibition was dose-dependent. The results suggested that the phosphopeptides have the potential to attenuate intestinal inflammation by oxidative stress.