Research on molecular mechanism of hepatic stem cell proliferation and development of mouse models for stem cell transplantation

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The first objective of this research project is clarification of molecular mechanisms in proliferation of primary mouse hepatic stem/progenitor cells (HSPCs). Primary mouse HSPCs were sorted using surface markers, and were infected with a viral vector expressing a target gene, then were cultured. Our data demonstrated that transcription factor Prox-1 enhances the proliferation of HSPCs, and Sall4 regulates the cell-fate determination of HSPCs. Second objective is development of mouse models for stem cell transplantation. The HSPCs were transplanted into liver-injured recipient mice. Our data showed that the recipient livers were reconstituted by donor HSPCs, and that donor cells were engrafted in long-term.