Establishment of vascular endothelial cell culture system using human placental cells

人胎盘细胞血管内皮细胞培养体系的建立

• 批准号: 20791174
• 负责人: MAKINO Hatsune
• 金额: $ 2.75万
• 依托单位: National Research Institute for Child Health and Development
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791174/
• 关键词: 生殖医療; 発生・分化; 発現制御; 発現抑制

This study was performed to isolate novel cells useful for prevention of various cardiovascular diseases and to develop clinical application of these cells. I demonstrated that cells derived from human amniotic mesoderm, chorionic plate, and villous chorion efficiently transdifferentiated into myotubes, and I demonstrated that human amniotic membrane-derived MSC had a high ability to transdifferentiate into cardiomyocytes. Moreover, I revealed that human placental artery-derived endothelial cells efficiently transdifferentiated into myoblasts/myocytes.

进行这项研究的目的是分离可用于预防各种心血管疾病的新细胞并开发这些细胞的临床应用。我证明了源自人羊膜中胚层、绒毛膜板和绒毛膜的细胞能够有效地转分化为肌管，并且我证明了源自人羊膜的MSC具有高度转分化为心肌细胞的能力。此外，我发现人胎盘动脉来源的内皮细胞有效地转分化为成肌细胞/肌细胞。

项目成果

Isolation and Culture of Human Mesenchymal Stem Cells.

人间充质干细胞的分离和培养。

• 发表时间: 2010
• 期刊: Organ Biology. 17(1)
• 作者: Umezawa A;Makino H.
• 通讯作者: Makino H.

Dystrophin conferral using human endothelium expressing HLA-E in the non-immunosuppressive murine model of Duchenne muscular dystrophy.

使用人内皮表达HLA-E的肌营养不良蛋白在Duchenne肌肉营养不良的非免疫抑制鼠模型中。

• DOI: 10.1093/hmg/ddq458
• 发表时间: 2011-01-15
• 期刊: Human molecular genetics
• 影响因子: 3.5
• 作者: Cui CH;Miyoshi S;Tsuji H;Makino H;Kanzaki S;Kami D;Terai M;Suzuki H;Umezawa A
• 通讯作者: Umezawa A

Xenografted Human Amniotic Membrane-Derived Mesenchymal Stem Cells Are Immunologically Tolerated and Transdifferentiated Into Cardiomyocytes

• DOI: 10.1161/circresaha.109.205260
• 发表时间: 2010-05-28
• 期刊: CIRCULATION RESEARCH
• 影响因子: 20.1
• 作者: Tsuji, Hiroko;Miyoshi, Shunichiro;Umezawa, Akihiro
• 通讯作者: Umezawa, Akihiro

細胞ソースの確立

建立细胞来源

• 发表时间: 2008
• 期刊: 日本臨床(特集:再生医学と医療-幹細胞の基礎研究と臨床の進歩) Vol.66、5巻
• 作者: 梅澤明弘;牧野初音
• 通讯作者: 牧野初音

Defining hypo-methylated regions of stem cell-specific promoters in human iPS cells derived from extra-embryonic amnions and lung fibroblasts.

• DOI: 10.1371/journal.pone.0013017
• 发表时间: 2010-09-27
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Nishino K;Toyoda M;Yamazaki-Inoue M;Makino H;Fukawatase Y;Chikazawa E;Takahashi Y;Miyagawa Y;Okita H;Kiyokawa N;Akutsu H;Umezawa A
• 通讯作者: Umezawa A