Single molecular analysis of the nuclear membrane formation

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The unique structure termed 'core region' is formed on the center of chromosome at telophase. In this study, I aimed to analyze the core formation mechanism at a single molecular level. To approach this issue, I investigated 1) the 3D-structure of emerin, and 2) a BAF binding site on the chromosomes, those of which were crucial factors to form the core region. By a nuclear magnetic resonance analysis, it was revealed that emerin did not take a solid structure in a solution except the LEM domain, which directly binds to BAF. On the other hand, BAF relatively binds to a transcription-active region at telophase although it binds to a transcription-negative region at interphase. From these evidences, the molecular dynamics of the proteins needed to form the core region are gradually uncovered at a single molecular level.